Panbio tests

 

• Abbott supplies Nasal, Nasopharingeal and IgG IgM tests
• Genprice supplies also the easy to use saliva tests.

How to use a covid test?

There are 5 types of Corona test kits

1. blood IgG IgM tests
2. saliva antigen tests for children
3. nasal tests for shops companies
4. nasopharingeal tests for doctors
5. pcr test for hospital labs

 

How to chose the best Corona test?

The best test is PCR but it is slow. It will take 3 hours to 24 hours to have the results. This is not option if you start school, shopping or a buisiness project.

all the other tests give results within 5 to 15 minites.

The worst test is the blood test. Why does this test exists anyway? because this was the first test developped in february 2020. The ealier antigen test was only developped in august 2020.

saliva test where the last kits developped in january 2021 and the are not better than nasal or nasopharingeal Panbio test from Abbott but they are very easy to use because completely panless. For example for babies covid testing saliva is preferred for the creche or kindergarden.

Tigsun Saliva Test

Genprice Gentaur

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zł837.76

SKU: TS-CoVS-25

Availability: In Stock

Bulk Pricing: Buy in bulk and save

Shipment: 1 or 2 Working Days for Free

Size: 25 Tests/Kit

Sensitivity: 92.59%

Specificity: 98.67%

Manual: Download PDF

Why self test are forbidden in the USA?

They where not forbidding in 2020. the FDA gave EUA emmergnecy use authorisations to a list of rapid self test for use in the USA under the Trump administation. From february 2021 the Biden administration started actively to forbid the use of self tests in the US. This is in big contrast with the situation in Germany and Austia where self tests are available in the Aldi. Lidl, REAL, Netto and other supermarkets. Austia made the Lepu antigen test kit madatody for schools and reduced the risk of teachers and caregivers by doing so.

In the USA self test are forbidden but guns lay in the supermarket. In Germany guns are forbidden but self tests lay in the supermarkt.

Prof. Dr. Lutzoni

How to test if someone is successfully vaccinated?

 

For airports, castings, concerts it is primordial to know if the attendants are vaccinated. A very fast and easy test has been developped in april 2021 to test this: the neutralising antibody test kit.

 

Whot of the 3 Neutralising test kits on blood should I use?

 

1.  IFA Immuno Fluorescent analysis tests with IFA analyser
2. visual collodial gold lateral flow tests with no istruments
3. ELISA test with ELISA reader

 

• The Collodial gold is the easiers to use, and ELISA the most expensive. Gentaur will supply ELISA readers at 1850 euro.
• An IFA analyser costs only 500 Euro from Gentaur
• an 96 well ELISA kit to run 44 samples in double will cost 240 euro
• an IFA will cost 85 euro for 25 cartridges

 

For Concerts and airporta the use of an IFA instrument at 500 euro is advised. The analysis is as accurate as with ELISA and will give on a scale from 1 to 10 the presence of protectng antibodies in the subject. It can be run in buk at the rate of 25 test an hour on blood per tester.

1. The traveler receives a disposable lancet and takes a blood sample in a capillary on his finger.
2. he will himself dispost his own blood on the cartridge with his name and number on the test table at the security check
3. the tester will put the cartridges into the IFA device and cal the protected numbers to pass the control station
4. the tester is responsible for the visible inspection if the subject uses his own blood on the numbered cartridge by a simple pensil

Measurement of SARS–CoV-2-specific antibody by IFA 

 

SARS-CoV-2 Rapid Antigen/ Tests for COVID-19 – Buyer’s Guide

Rapid antigen Test Method

COVID Self Antigen Tests PANBIO

aBBOTT SARS-CoV-2 antigen, using saliva or swab samples are now widely available.

These SELF tests are less sensitive than PCR but easy to use and interpret, time to result can be between 10 minutes to 40 minutes depending on the type of assay.

Types of Covid antigen detection kits:

1. chemiluminescence immunoassay (CLIA)
2. fluorescent (FIA) immunoassays
3. lateral flow immunoassays (LFIA)
4. lateral flow fluorescent immunoassays

Antigen test kits that target this protein are less likely to give false-positives.

For saliva samples have been deployed at many international airports to screen individuals for SARS-CoV-2.

Immunochromatographic/Lateral-Flow Immunoassays (LFIA):

• This is based on the design of the common pregnancy test. The test is a strip containing immobilised reagents; specially-coated antibodies, negative and positive controls, and a window to view the result. The result shows in the form of a coloured line, that must be viewed at a specified time-point.
• There are also commercialized automatic reading devices, that the diagnostic strip is inserted into, thus avoiding human error. These readers have performed better in validation studies than visual reading. These readers can give a semi-quantitative or quantitative result. These tests can be performed by unskilled operators in near-patient settings and turnaround times are usually 15 minutes. This makes them ideal for ports but only if passenger levels are reduced, as there is a chance of false-negatives due to low viral load in the patient not been detected.

COVID-19 Nucleic-Acid/Molecular Near-Patient Testing/Point-of-Care Testing:

• COVID-19 tests that use isothermal nucleic acid amplification technology to detect SARS-CoV-2 RNA have shown it to be less accurate than RT-PCR. However, this lower level of sensitivity may be due to improper procedure; using viral transport media instead of inserting swabs directly, while other evidence shows increased accuracy with an extra RNA extraction step before inserting swab.
• Other variations of this technology include nicking enzyme-assisted reaction (NEAR), recombinase polymerase amplification (RPA) and Insulated Isothermal Polymerase Chain Reaction (RT-iiPCR).

CRISPR & isothermal amplification:

CRISPR SARS-CoV-2 tests on the market use the isothermal amplification method. But its use of proprietary molecular tools; SHERLOCK and DETECTR tools to amplify the sequence in combination with the CRISPR Cas 12/13 system for detection have demonstrated similar results to that of PCR. Its sensitivity depends on the type of readout, with fluorescence in the lab being superior to lateral-flow readouts.

Sequencing & Isothermal amplification:

One problem with isothermal amplification techniques is that substances present in the sample might interfere with the color change or turbidity, which is interpreted into a result. The use of nano-sequencing to read the results can increase sensitivity and specificity. An example of this test is the LAMPORE assay developed at Oxford University.

Abbott IdNow Rapid PCR

IdNow rapid PCR tests are available (FDA-EUA and CE-IVD) and are designed for near-patient settings, with some already in use at airports. These PCR cyclers have a small footprint and typically weigh 1Kg, they analyze swab samples and automate the nucleic acid extraction and amplification, with only basic skills needed to operate. Average turnaround time to result is 40 minutes.