Molecular characterization and phylogenetic analysis of Fasciola hepatica from high- plateau and steppe areas in Algeria

A previous study based on mitochondrial DNA markers reported Fasciola hepatica in Algeria. However, the exact species identification is still required. In this report, a total of 68 isolates Fasciola, gathered from high-plateau (Bordj-Bou-Arreridj) and steppes (Djelfa) areas of Algeria, identified at the species level by multiplex PCR and PCR-restriction fragment length polymorphism (RFLP) for carboxykinase nuclear phosphoenolpyruvate (PEPCK) and DNA polymerase delta (pold), respectively. Multiplex PCR results contrary to that of PCR-RFLP; However, the next nucleotide sequencing PEPCK clearly showed that all isolates should be classified as F. hepatica. Both markers of mitochondria, NADH dehydrogenase subunit I (nad1) and cytochrome c oxidase subunit 1 (COX1), revealed a close relationship between the parasite populations of the highlands and the people of the savannah. A direction of the spread of highland pasture is indicated as a former resident of the more diversified than the last. In addition, the population is more closely related to the population of Spain than those of Egypt or Afghanistan. Given the characteristics of the population of F. hepatica in Spain and the history of the cattle trade, it seems likely that the parasite was introduced into Algeria from Europe via the Mediterranean sea.We current pandemic of COVID-19, which is caused by spillover from animal-derived human coronavirus occurred at Wuhan region of China in December 2019. China, the virus has spread to 188 countries and territories around the world, reached the Sahel region on March 2, 2020. Since the whole-genome sequencing (WGS) data is very important to understand the dynamics of the spread of the pandemic in progress but only limited data available from the Sahel region sequenced to date, we have focused our efforts on producing the first Mali sequencing data available. Screening Mali 217 patient samples for the presence of SARS-CoV-2 resulted in 38 positive isolates, of which 21 whole genome sequences generated. Our analysis shows that both A beginning (19B) and then observed B (20A / C) clade were present in Mali, showed few and independent introductions of SARS-CoV-2 to the Sahel region.

Molecular phylogenetic and genetic variability Fasciola gigantica in the province of Kermanshah, western Iran with a view to understanding the haplotype distribution in Asia and Africa

Over the past decade, diagnostic tools to detect and differentiate Fasciola species has improved, but our understanding of the haplotype distribution and population structure of the parasite is less clear. This study was designed to survey this gap in F. gigantica epidemiology in Kermanshah province, western Iran from 2015 to 2017. Sixty-eight Fasciola isolates collected from slaughterhouses from the province. We evaluated test PCR-RFLP of ITS1 gene for identification of species Fasciola using enzymes RsaI. After the identification of species Fasciola, partial sequence of mitochondrial NADH dehydrogenase subunit 1 (ND1) gene F. gigantica used for the subsequent development of the tree and phylogenetic analyzes network. PRFLP PCR-based pro fi le, one (6.25%) of the isolates of sheep and 19 (39.60%) of the isolates were detected as F. gigantica livestock, while 93.75% of the isolates sheep, 60.40% of the isolates cattle and goats are all F. hepatica isolates. In 20 worms were analyzed, five ND1 haplotypes were detected. Statistically significant genetic differences shown between the Iranian population and all other populations. Evidence is presented for the existence of two separate populations: African and West Asian Worms Worms gigantica gigantica and East Asia. Genetic relationship between haplotype associated with geographic divisions.

 

probe molecule, and analsysis phylogenetic isolation of Coxiella burnetii from aborted fetuses and ticks

Q fever is a zoonotic infection threaten human health, causes abortion in cattle, sheep and goats. Coxiella burnetii (C. burnetii) also lead to serious problems such as low birth weight, infertility. This study is the first example for the analysis of Q fever in the area around the Black Sea in Turkey. In the study, 270 of aborted fetuses (171 cows, 79 goats, 20 goats) and 1069 tick samples intended to be sought by PCR. C. burnetii DNA was detected in 8 (2.96%) of 270 specimens of sheep while it was not to be found in cows and goats specimens. 406 sample pool of 1069 created tick samples (490 men, 579 women) were collected from 254 cattle (187 cows, 54 goats, 13 goats) and 11 of them tested positive. Tick ​​species are defined as positive C. burnetii Hyalomma marginatum, Hyalomma anoliticum excavatum, Hyalomma detritum and Boophilus annulatus. Agent isolation done in embryonated eggs.

rno-let-7a Primers
MP-r00033	ABM	150 ul / 10 uM	EUR 211.2

mmu-let-7a Primers
MPM00007	ABM	150 ul / 10 uM	EUR 145.2

Mouse pre-microRNA Expression Construct let-7a-1
MMIR-let-7a-1-PA-1	SBI	Bacterial Streak	EUR 820.8

Mouse pre-microRNA Expression Construct let-7a-2
MMIR-let-7a-2-PA-1	SBI	Bacterial Streak	EUR 820.8

mmu-let-7a-5p Primers
MPM00736	ABM	150 ul / 10 uM	EUR 145.2

rno-let-7a-5p Primers
MPR00307	ABM	150 ul / 10 uM	EUR 211.2

hsa-let-7a miRNA Antagomir
MNH01000	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7a miRNA Inhibitor
MIH01000	ABM	2 x 5.0 nmol	EUR 211.2

pLL3.7- Hsa- Let- 7a- 1miRNA
PVT10410	Lifescience Market	2 ug	EUR 361.2

hsa-let-7b Primers
MPH01007	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7c Primers
MPH01008	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7d Primers
MPH01009	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7e Primers
MPH01010	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7f Primers
MPH01011	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7g Primers
MPH01012	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7i Primers
MPH01013	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7a-1-3p Primers
MPM00734	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7a-2-3p Primers
MPM00735	ABM	150 ul / 10 uM	EUR 145.2

rno-let-7a-2-3p Primers
MPR00306	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7a-1-3p Primers
MPR00878	ABM	150 ul / 10 uM	EUR 211.2

hsa-let-7a-3p miRNA Antagomir
MNH01002	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7a-5p miRNA Antagomir
MNH01003	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7a-3p miRNA Inhibitor
MIH01002	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7a-5p miRNA Inhibitor
MIH01003	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7b-3p Primers
MPH02043	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7b-5p Primers
MPH02044	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7c-3p Primers
MPH02045	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7c-5p Primers
MPH02046	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7d-3p Primers
MPH02047	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7d-5p Primers
MPH02048	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7e-3p Primers
MPH02049	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7e-5p Primers
MPH02050	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7f-5p Primers
MPH02053	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7g-3p Primers
MPH02054	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7g-5p Primers
MPH02055	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7i-3p Primers
MPH02056	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7i-5p Primers
MPH02057	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7a RT-PCR Detection Kit
20-abx096015	Abbexa	EUR 644.40 EUR 878.40 EUR 477.60	100 rxns 200 rxns 50 rxns

hsa-let-7a RT-PCR Primer Set
abx096769-1nmol	Abbexa	1 nmol	EUR 343.2

hsa-let-7a-2-3p miRNA Antagomir
MNH01001	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7a-2-3p miRNA Inhibitor
MIH01001	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7f-1-3p Primers
MPH02051	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7f-2-3p Primers
MPH02052	ABM	150 ul / 10 uM	EUR 145.2

ICP Quality Control Standard 7A
QC-7A	Scientific Laboratory Supplies	125ML	EUR 169.86

rno-let-7a miRNA Antagomir
MNR01000	ABM	2 x 5.0 nmol	EUR 394.8

rno-let-7a miRNA Inhibitor
MIR01000	ABM	2 x 5.0 nmol	EUR 211.2

mmu-let-7a miRNA Antagomir
MNM01000	ABM	2 x 5.0 nmol	EUR 394.8

mmu-let-7a miRNA Inhibitor
MIM01000	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7a RT-PCR Detection and U6 Calibration Kit
20-abx096392	Abbexa	EUR 828.00 EUR 1144.80 EUR 594.00	100 rxns 200 rxns 50 rxns

ICP Qualicty Control Standard 7A
QC-7A-500	Scientific Laboratory Supplies	500ML	EUR 281.58

rno-let-7d Primers
MP-r00007	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7b Primers
MP-r00034	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7c Primers
MP-r00035	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7e Primers
MP-r00036	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7f Primers
MP-r00037	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7i Primers
MP-r00038	ABM	150 ul / 10 uM	EUR 211.2

mmu-let-7b Primers
MPM00008	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7c Primers
MPM00009	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7d Primers
MPM00010	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7e Primers
MPM00011	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7f Primers
MPM00012	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7g Primers
MPM00013	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7i Primers
MPM00014	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7j Primers
MPM00753	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7k Primers
MPM00754	ABM	150 ul / 10 uM	EUR 145.2

rno-let-7a-5p miRNA Antagomir
MNR01003	ABM	2 x 5.0 nmol	EUR 394.8

rno-let-7a-5p miRNA Inhibitor
MIR01003	ABM	2 x 5.0 nmol	EUR 211.2

mmu-let-7a-5p miRNA Antagomir
MNM01003	ABM	2 x 5.0 nmol	EUR 394.8

mmu-let-7a-5p miRNA Inhibitor
MIM01003	ABM	2 x 5.0 nmol	EUR 211.2

rno-miR-7a Primers
MP-r00026	ABM	150 ul / 10 uM	EUR 211.2

rno-miR-7a Primers
MP-r00027	ABM	150 ul / 10 uM	EUR 211.2

mmu-miR-7a Primers
MPM00701	ABM	150 ul / 10 uM	EUR 145.2

rno-let-7a-1-3p miRNA Antagomir
MNR01001	ABM	2 x 5.0 nmol	EUR 394.8

rno-let-7a-2-3p miRNA Antagomir
MNR01002	ABM	2 x 5.0 nmol	EUR 394.8

rno-let-7a-1-3p miRNA Inhibitor
MIR01001	ABM	2 x 5.0 nmol	EUR 211.2

rno-let-7a-2-3p miRNA Inhibitor
MIR01002	ABM	2 x 5.0 nmol	EUR 211.2

mmu-let-7a-1-3p miRNA Antagomir
MNM01001	ABM	2 x 5.0 nmol	EUR 394.8

mmu-let-7a-2-3p miRNA Antagomir
MNM01002	ABM	2 x 5.0 nmol	EUR 394.8

mmu-let-7a-1-3p miRNA Inhibitor
MIM01001	ABM	2 x 5.0 nmol	EUR 211.2

mmu-let-7a-2-3p miRNA Inhibitor
MIM01002	ABM	2 x 5.0 nmol	EUR 211.2

mmu-let-7b-3p Primers
MPM00737	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7b-5p Primers
MPM00738	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7c-5p Primers
MPM00741	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7d-3p Primers
MPM00742	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7d-5p Primers
MPM00743	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7e-3p Primers
MPM00744	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7e-5p Primers
MPM00745	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7f-5p Primers
MPM00748	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7g-3p Primers
MPM00749	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7g-5p Primers
MPM00750	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7i-3p Primers
MPM00751	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7i-5p Primers
MPM00752	ABM	150 ul / 10 uM	EUR 145.2

rno-let-7b-3p Primers
MPR00308	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7b-5p Primers
MPR00309	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7c-5p Primers
MPR00312	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7d-3p Primers
MPR00313	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7d-5p Primers
MPR00314	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7e-3p Primers
MPR00315	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7e-5p Primers
MPR00316	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7f-5p Primers
MPR00319	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7g-3p Primers
MPR00320	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7g-5p Primers
MPR00321	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7i-3p Primers
MPR00322	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7i-5p Primers
MPR00323	ABM	150 ul / 10 uM	EUR 211.2

Dinitrophenyl (DNP)-HSA protein Conjugate
AV-9330-HSA	Alpha Diagnostics	10 mg	EUR 343.2

hsa-let-7b miRNA Antagomir
MNH01004	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7c miRNA Antagomir
MNH01007	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7d miRNA Antagomir
MNH01010	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7e miRNA Antagomir
MNH01013	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7f miRNA Antagomir
MNH01016	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7g miRNA Antagomir
MNH01020	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7i miRNA Antagomir
MNH01023	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7b miRNA Inhibitor
MIH01004	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7c miRNA Inhibitor
MIH01007	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7d miRNA Inhibitor
MIH01010	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7e miRNA Inhibitor
MIH01013	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7g miRNA Inhibitor
MIH01020	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7i miRNA Inhibitor
MIH01023	ABM	2 x 5.0 nmol	EUR 211.2

mmu-miR-7a-5p Primers
MPM02196	ABM	150 ul / 10 uM	EUR 145.2

rno-miR-7a-5p Primers
MPR00838	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7a-1* RT-PCR Detection Kit
20-abx096331	Abbexa	EUR 644.40 EUR 878.40 EUR 477.60	100 rxns 200 rxns 50 rxns

rno-let-7a-1* RT-PCR Primer Set
abx097085-1nmol	Abbexa	1 nmol	EUR 343.2

Human pre-microRNA Expression Construct let-7a-1
PMIRHlet7a1PA-1	SBI	Bacterial Streak	EUR 820.8

Human pre-microRNA Expression Construct let-7a-2
PMIRHlet7a2PA-1	SBI	Bacterial Streak	EUR 820.8

Human pre-microRNA Expression Construct let-7a-3
PMIRHlet7a3PA-1	SBI	Bacterial Streak	EUR 820.8

mmu-let-7c-1-3p Primers
MPM00739	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7c-2-3p Primers
MPM00740	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7f-1-3p Primers
MPM00746	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7f-2-3p Primers
MPM00747	ABM	150 ul / 10 uM	EUR 145.2

rno-let-7c-1-3p Primers
MPR00310	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7c-2-3p Primers
MPR00311	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7f-1-3p Primers
MPR00317	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7f-2-3p Primers
MPR00318	ABM	150 ul / 10 uM	EUR 211.2

hsa-let-7b-3p miRNA Antagomir
MNH01005	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7b-5p miRNA Antagomir
MNH01006	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7c-3p miRNA Antagomir
MNH01008	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7c-5p miRNA Antagomir
MNH01009	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7d-3p miRNA Antagomir
MNH01011	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7d-5p miRNA Antagomir
MNH01012	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7e-3p miRNA Antagomir
MNH01014	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7e-5p miRNA Antagomir
MNH01015	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7f-5p miRNA Antagomir
MNH01019	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7g-3p miRNA Antagomir
MNH01021	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7g-5p miRNA Antagomir
MNH01022	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7i-3p miRNA Antagomir
MNH01024	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7i-5p miRNA Antagomir
MNH01025	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7b-3p miRNA Inhibitor
MIH01005	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7b-5p miRNA Inhibitor
MIH01006	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7c-3p miRNA Inhibitor
MIH01008	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7c-5p miRNA Inhibitor
MIH01009	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7d-3p miRNA Inhibitor
MIH01011	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7d-5p miRNA Inhibitor
MIH01012	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7e-3p miRNA Inhibitor
MIH01014	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7e-5p miRNA Inhibitor
MIH01015	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7f-5p miRNA Inhibitor
MIH01019	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7g-3p miRNA Inhibitor
MIH01021	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7g-5p miRNA Inhibitor
MIH01022	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7i-3p miRNA Inhibitor
MIH01024	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7i-5p miRNA Inhibitor
MIH01025	ABM	2 x 5.0 nmol	EUR 211.2

Mouse pre-microRNA Expression Construct let-7b
MMIR-let-7b-PA-1	SBI	Bacterial Streak	EUR 820.8

Mouse pre-microRNA Expression Construct let-7d
MMIR-let-7d-PA-1	SBI	Bacterial Streak	EUR 820.8

Mouse pre-microRNA Expression Construct let-7e
MMIR-let-7e-PA-1	SBI	Bacterial Streak	EUR 820.8

Mouse pre-microRNA Expression Construct let-7g
MMIR-let-7g-PA-1	SBI	Bacterial Streak	EUR 820.8

hsa-let-7a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097603	Abbexa	EUR 878.40 EUR 1245.60 EUR 627.60	100 rxns 200 rxns 50 rxns

mmu-miR-7a-1-3p Primers
MPM02194	ABM	150 ul / 10 uM	EUR 145.2

mmu-miR-7a-2-3p Primers
MPM02195	ABM	150 ul / 10 uM	EUR 145.2

rno-miR-7a-1-3p Primers
MPR00836	ABM	150 ul / 10 uM	EUR 211.2

rno-miR-7a-2-3p Primers
MPR00837	ABM	150 ul / 10 uM	EUR 211.2

hsa-let-7b RT-PCR Detection Kit
20-abx096016	Abbexa	EUR 644.40 EUR 878.40 EUR 477.60	100 rxns 200 rxns 50 rxns

hsa-let-7c RT-PCR Detection Kit
20-abx096017	Abbexa	EUR 644.40 EUR 878.40 EUR 477.60	100 rxns 200 rxns 50 rxns

hsa-let-7d RT-PCR Detection Kit
20-abx096018	Abbexa	EUR 644.40 EUR 878.40 EUR 477.60	100 rxns 200 rxns 50 rxns

hsa-let-7e RT-PCR Detection Kit
20-abx096019	Abbexa	EUR 644.40 EUR 878.40 EUR 477.60	100 rxns 200 rxns 50 rxns

hsa-let-7f RT-PCR Detection Kit
20-abx096020	Abbexa	EUR 644.40 EUR 878.40 EUR 477.60	100 rxns 200 rxns 50 rxns

hsa-let-7g RT-PCR Detection Kit
20-abx096021	Abbexa	EUR 644.40 EUR 878.40 EUR 477.60	100 rxns 200 rxns 50 rxns

hsa-let-7i RT-PCR Detection Kit
20-abx096022	Abbexa	EUR 644.40 EUR 878.40 EUR 477.60	100 rxns 200 rxns 50 rxns

hsa-let-7f RT-PCR Detection Kit
20-abx096023	Abbexa	EUR 644.40 EUR 878.40 EUR 477.60	100 rxns 200 rxns 50 rxns

Agents were stained with Giemsa and shows. sequence analysis performed for TUR / SAM / coxiella_1 (MN917207) isolates and the phylogenetic tree was created. This tree, made in accordance with the IS1111 transposon gene, do not form a distinct branch in terms of affiliation host (goats, sheep, tick, human beings) and geographical distribution. As a result, an important zoonotic agent, C. burnetii diagnosed in sheep fetus aborted and infections have proven to have spread among sheep herd in the Black Sea region. Additionally, four separate tick species found in our region and discovered an important host agent for infection.