Molecular characterization and phylogenetic analysis of Fasciola hepatica from high- plateau and steppe areas in Algeria

A previous study based on mitochondrial DNA markers reported Fasciola hepatica in Algeria. However, the exact species identification is still required. In this report, a total of 68 isolates Fasciola, gathered from high-plateau (Bordj-Bou-Arreridj) and steppes (Djelfa) areas of Algeria, identified at the species level by multiplex PCR and PCR-restriction fragment length polymorphism (RFLP) for carboxykinase nuclear phosphoenolpyruvate (PEPCK) and DNA polymerase delta (pold), respectively. Multiplex PCR results contrary to that of PCR-RFLP; However, the next nucleotide sequencing PEPCK clearly showed that all isolates should be classified as F. hepatica. Both markers of mitochondria, NADH dehydrogenase subunit I (nad1) and cytochrome c oxidase subunit 1 (COX1), revealed a close relationship between the parasite populations of the highlands and the people of the savannah. A direction of the spread of highland pasture is indicated as a former resident of the more diversified than the last. In addition, the population is more closely related to the population of Spain than those of Egypt or Afghanistan. Given the characteristics of the population of F. hepatica in Spain and the history of the cattle trade, it seems likely that the parasite was introduced into Algeria from Europe via the Mediterranean sea.We current pandemic of COVID-19, which is caused by spillover from animal-derived human coronavirus occurred at Wuhan region of China in December 2019. China, the virus has spread to 188 countries and territories around the world, reached the Sahel region on March 2, 2020. Since the whole-genome sequencing (WGS) data is very important to understand the dynamics of the spread of the pandemic in progress but only limited data available from the Sahel region sequenced to date, we have focused our efforts on producing the first Mali sequencing data available. Screening Mali 217 patient samples for the presence of SARS-CoV-2 resulted in 38 positive isolates, of which 21 whole genome sequences generated. Our analysis shows that both A beginning (19B) and then observed B (20A / C) clade were present in Mali, showed few and independent introductions of SARS-CoV-2 to the Sahel region.

Molecular phylogenetic and genetic variability Fasciola gigantica in the province of Kermanshah, western Iran with a view to understanding the haplotype distribution in Asia and Africa

Over the past decade, diagnostic tools to detect and differentiate Fasciola species has improved, but our understanding of the haplotype distribution and population structure of the parasite is less clear. This study was designed to survey this gap in F. gigantica epidemiology in Kermanshah province, western Iran from 2015 to 2017. Sixty-eight Fasciola isolates collected from slaughterhouses from the province. We evaluated test PCR-RFLP of ITS1 gene for identification of species Fasciola using enzymes RsaI. After the identification of species Fasciola, partial sequence of mitochondrial NADH dehydrogenase subunit 1 (ND1) gene F. gigantica used for the subsequent development of the tree and phylogenetic analyzes network. PRFLP PCR-based pro fi le, one (6.25%) of the isolates of sheep and 19 (39.60%) of the isolates were detected as F. gigantica livestock, while 93.75% of the isolates sheep, 60.40% of the isolates cattle and goats are all F. hepatica isolates. In 20 worms were analyzed, five ND1 haplotypes were detected. Statistically significant genetic differences shown between the Iranian population and all other populations. Evidence is presented for the existence of two separate populations: African and West Asian Worms Worms gigantica gigantica and East Asia. Genetic relationship between haplotype associated with geographic divisions.

 

probe molecule, and analsysis phylogenetic isolation of Coxiella burnetii from aborted fetuses and ticks

Q fever is a zoonotic infection threaten human health, causes abortion in cattle, sheep and goats. Coxiella burnetii (C. burnetii) also lead to serious problems such as low birth weight, infertility. This study is the first example for the analysis of Q fever in the area around the Black Sea in Turkey. In the study, 270 of aborted fetuses (171 cows, 79 goats, 20 goats) and 1069 tick samples intended to be sought by PCR. C. burnetii DNA was detected in 8 (2.96%) of 270 specimens of sheep while it was not to be found in cows and goats specimens. 406 sample pool of 1069 created tick samples (490 men, 579 women) were collected from 254 cattle (187 cows, 54 goats, 13 goats) and 11 of them tested positive. Tick ​​species are defined as positive C. burnetii Hyalomma marginatum, Hyalomma anoliticum excavatum, Hyalomma detritum and Boophilus annulatus. Agent isolation done in embryonated eggs.

hsa-let-7b Primers
MPH01007	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7c Primers
MPH01008	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7d Primers
MPH01009	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7e Primers
MPH01010	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7f Primers
MPH01011	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7g Primers
MPH01012	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7i Primers
MPH01013	ABM	150 ul / 10 uM	EUR 145.2

rno-let-7a Primers
MP-r00033	ABM	150 ul / 10 uM	EUR 211.2

mmu-let-7a Primers
MPM00007	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7b-3p Primers
MPH02043	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7b-5p Primers
MPH02044	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7c-3p Primers
MPH02045	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7c-5p Primers
MPH02046	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7d-3p Primers
MPH02047	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7d-5p Primers
MPH02048	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7e-3p Primers
MPH02049	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7e-5p Primers
MPH02050	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7f-5p Primers
MPH02053	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7g-3p Primers
MPH02054	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7g-5p Primers
MPH02055	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7i-3p Primers
MPH02056	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7i-5p Primers
MPH02057	ABM	150 ul / 10 uM	EUR 145.2

rno-let-7a-5p Primers
MPR00307	ABM	150 ul / 10 uM	EUR 211.2

mmu-let-7a-5p Primers
MPM00736	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7f-1-3p Primers
MPH02051	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7f-2-3p Primers
MPH02052	ABM	150 ul / 10 uM	EUR 145.2

rno-let-7a-1-3p Primers
MPR00878	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7a-2-3p Primers
MPR00306	ABM	150 ul / 10 uM	EUR 211.2

mmu-let-7a-1-3p Primers
MPM00734	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7a-2-3p Primers
MPM00735	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7a RT-PCR Primer Set
abx096769-1nmol	Abbexa	1 nmol	EUR 343.2

rno-let-7d Primers
MP-r00007	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7b Primers
MP-r00034	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7c Primers
MP-r00035	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7e Primers
MP-r00036	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7f Primers
MP-r00037	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7i Primers
MP-r00038	ABM	150 ul / 10 uM	EUR 211.2

mmu-let-7b Primers
MPM00008	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7c Primers
MPM00009	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7d Primers
MPM00010	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7e Primers
MPM00011	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7f Primers
MPM00012	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7g Primers
MPM00013	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7i Primers
MPM00014	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7j Primers
MPM00753	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7k Primers
MPM00754	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-1 Primers
MPH01014	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-7 Primers
MPH01976	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-9 Primers
MPH02015	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-16 Primers
MPH01191	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-17 Primers
MPH01192	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-21 Primers
MPH01259	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-22 Primers
MPH01277	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-24 Primers
MPH01294	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-25 Primers
MPH01295	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-31 Primers
MPH01329	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-93 Primers
MPH02022	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-95 Primers
MPH02035	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-96 Primers
MPH02036	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-98 Primers
MPH02037	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-32 Primers
MPH01417	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-100 Primers
MPH01015	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-101 Primers
MPH01016	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-103 Primers
MPH01017	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-105 Primers
MPH01019	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-107 Primers
MPH01022	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-10a Primers
MPH01023	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-10b Primers
MPH01024	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-122 Primers
MPH01045	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-124 Primers
MPH01060	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-126 Primers
MPH01082	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-128 Primers
MPH01110	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-132 Primers
MPH01144	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-134 Primers
MPH01151	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-136 Primers
MPH01154	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-137 Primers
MPH01155	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-138 Primers
MPH01156	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-141 Primers
MPH01161	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-143 Primers
MPH01164	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-144 Primers
MPH01165	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-145 Primers
MPH01166	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-147 Primers
MPH01172	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-149 Primers
MPH01178	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-150 Primers
MPH01179	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-152 Primers
MPH01182	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-153 Primers
MPH01183	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-154 Primers
MPH01187	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-155 Primers
MPH01188	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-15a Primers
MPH01189	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-15b Primers
MPH01190	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-182 Primers
MPH01197	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-183 Primers
MPH01201	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-184 Primers
MPH01202	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-185 Primers
MPH01203	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-186 Primers
MPH01204	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-187 Primers
MPH01205	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-18a Primers
MPH01208	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-18b Primers
MPH01209	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-190 Primers
MPH01210	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-191 Primers
MPH01214	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-192 Primers
MPH01221	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-194 Primers
MPH01225	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-195 Primers
MPH01226	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-197 Primers
MPH01229	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-198 Primers
MPH01238	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-19a Primers
MPH01242	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-19b Primers
MPH01243	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-202 Primers
MPH01247	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-203 Primers
MPH01248	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-204 Primers
MPH01249	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-205 Primers
MPH01250	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-206 Primers
MPH01254	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-20a Primers
MPH01257	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-20b Primers
MPH01258	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-210 Primers
MPH01260	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-211 Primers
MPH01261	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-212 Primers
MPH01268	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-214 Primers
MPH01269	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-215 Primers
MPH01270	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-218 Primers
MPH01273	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-221 Primers
MPH01281	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-222 Primers
MPH01282	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-223 Primers
MPH01283	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-224 Primers
MPH01284	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-23a Primers
MPH01291	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-23b Primers
MPH01292	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-23c Primers
MPH01293	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-26a Primers
MPH01296	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-26b Primers
MPH01297	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-27a Primers
MPH01298	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-27b Primers
MPH01299	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-297 Primers
MPH01306	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-298 Primers
MPH01307	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-29a Primers
MPH01310	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-29b Primers
MPH01311	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-29c Primers
MPH01312	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-300 Primers
MPH01313	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-30a Primers
MPH01324	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-30b Primers
MPH01325	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-30c Primers
MPH01326	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-30d Primers
MPH01327	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-30e Primers
MPH01328	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-503 Primers
MPH01743	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-504 Primers
MPH01744	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-505 Primers
MPH01745	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-506 Primers
MPH01746	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-507 Primers
MPH01747	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-510 Primers
MPH01753	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-511 Primers
MPH01754	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-514 Primers
MPH01761	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-521 Primers
MPH01795	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-522 Primers
MPH01796	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-523 Primers
MPH01797	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-527 Primers
MPH01803	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-539 Primers
MPH01806	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-541 Primers
MPH01807	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-543 Primers
MPH01810	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-544 Primers
MPH01811	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-545 Primers
MPH01813	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-549 Primers
MPH01844	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-552 Primers
MPH01849	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-553 Primers
MPH01850	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-554 Primers
MPH01851	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-555 Primers
MPH01852	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-557 Primers
MPH01855	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-558 Primers
MPH01856	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-559 Primers
MPH01857	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-561 Primers
MPH01858	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-562 Primers
MPH01859	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-563 Primers
MPH01860	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-564 Primers
MPH01861	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-566 Primers
MPH01862	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-567 Primers
MPH01863	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-568 Primers
MPH01864	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-569 Primers
MPH01865	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-570 Primers
MPH01866	ABM	150 ul / 10 uM	EUR 145.2

Agents were stained with Giemsa and shows. sequence analysis performed for TUR / SAM / coxiella_1 (MN917207) isolates and the phylogenetic tree was created. This tree, made in accordance with the IS1111 transposon gene, do not form a distinct branch in terms of affiliation host (goats, sheep, tick, human beings) and geographical distribution. As a result, an important zoonotic agent, C. burnetii diagnosed in sheep fetus aborted and infections have proven to have spread among sheep herd in the Black Sea region. Additionally, four separate tick species found in our region and discovered an important host agent for infection.