A previous study based on mitochondrial DNA markers reported Fasciola hepatica in Algeria. However, the exact species identification is still required. In this report, a total of 68 isolates Fasciola, gathered from high-plateau (Bordj-Bou-Arreridj) and steppes (Djelfa) areas of Algeria, identified at the species level by multiplex PCR and PCR-restriction fragment length polymorphism (RFLP) for carboxykinase nuclear phosphoenolpyruvate (PEPCK) and DNA polymerase delta (pold), respectively. Multiplex PCR results contrary to that of PCR-RFLP; However, the next nucleotide sequencing PEPCK clearly showed that all isolates should be classified as F. hepatica. Both markers of mitochondria, NADH dehydrogenase subunit I (nad1) and cytochrome c oxidase subunit 1 (COX1), revealed a close relationship between the parasite populations of the highlands and the people of the savannah. A direction of the spread of highland pasture is indicated as a former resident of the more diversified than the last. In addition, the population is more closely related to the population of Spain than those of Egypt or Afghanistan. Given the characteristics of the population of F. hepatica in Spain and the history of the cattle trade, it seems likely that the parasite was introduced into Algeria from Europe via the Mediterranean sea.We current pandemic of COVID-19, which is caused by spillover from animal-derived human coronavirus occurred at Wuhan region of China in December 2019. China, the virus has spread to 188 countries and territories around the world, reached the Sahel region on March 2, 2020. Since the whole-genome sequencing (WGS) data is very important to understand the dynamics of the spread of the pandemic in progress but only limited data available from the Sahel region sequenced to date, we have focused our efforts on producing the first Mali sequencing data available. Screening Mali 217 patient samples for the presence of SARS-CoV-2 resulted in 38 positive isolates, of which 21 whole genome sequences generated. Our analysis shows that both A beginning (19B) and then observed B (20A / C) clade were present in Mali, showed few and independent introductions of SARS-CoV-2 to the Sahel region.

Molecular phylogenetic and genetic variability Fasciola gigantica in the province of Kermanshah, western Iran with a view to understanding the haplotype distribution in Asia and Africa

Over the past decade, diagnostic tools to detect and differentiate Fasciola species has improved, but our understanding of the haplotype distribution and population structure of the parasite is less clear. This study was designed to survey this gap in F. gigantica epidemiology in Kermanshah province, western Iran from 2015 to 2017. Sixty-eight Fasciola isolates collected from slaughterhouses from the province. We evaluated test PCR-RFLP of ITS1 gene for identification of species Fasciola using enzymes RsaI. After the identification of species Fasciola, partial sequence of mitochondrial NADH dehydrogenase subunit 1 (ND1) gene F. gigantica used for the subsequent development of the tree and phylogenetic analyzes network. PRFLP PCR-based pro fi le, one (6.25%) of the isolates of sheep and 19 (39.60%) of the isolates were detected as F. gigantica livestock, while 93.75% of the isolates sheep, 60.40% of the isolates cattle and goats are all F. hepatica isolates. In 20 worms were analyzed, five ND1 haplotypes were detected. Statistically significant genetic differences shown between the Iranian population and all other populations. Evidence is presented for the existence of two separate populations: African and West Asian Worms Worms gigantica gigantica and East Asia. Genetic relationship between haplotype associated with geographic divisions.

 Molecular characterization and phylogenetic analysis of Fasciola hepatica from high- plateau and steppe areas in Algeria
Molecular characterization and phylogenetic analysis of Fasciola hepatica from high- plateau and steppe areas in Algeria

 

probe molecule, and analsysis phylogenetic isolation of Coxiella burnetii from aborted fetuses and ticks

Q fever is a zoonotic infection threaten human health, causes abortion in cattle, sheep and goats. Coxiella burnetii (C. burnetii) also lead to serious problems such as low birth weight, infertility. This study is the first example for the analysis of Q fever in the area around the Black Sea in Turkey. In the study, 270 of aborted fetuses (171 cows, 79 goats, 20 goats) and 1069 tick samples intended to be sought by PCR. C. burnetii DNA was detected in 8 (2.96%) of 270 specimens of sheep while it was not to be found in cows and goats specimens. 406 sample pool of 1069 created tick samples (490 men, 579 women) were collected from 254 cattle (187 cows, 54 goats, 13 goats) and 11 of them tested positive. Tick ​​species are defined as positive C. burnetii Hyalomma marginatum, Hyalomma anoliticum excavatum, Hyalomma detritum and Boophilus annulatus. Agent isolation done in embryonated eggs.

hsa-let-7b Primers

MPH01007 150 ul / 10 uM
EUR 145.2

hsa-let-7c Primers

MPH01008 150 ul / 10 uM
EUR 145.2

hsa-let-7d Primers

MPH01009 150 ul / 10 uM
EUR 145.2

hsa-let-7e Primers

MPH01010 150 ul / 10 uM
EUR 145.2

hsa-let-7f Primers

MPH01011 150 ul / 10 uM
EUR 145.2

hsa-let-7g Primers

MPH01012 150 ul / 10 uM
EUR 145.2

hsa-let-7i Primers

MPH01013 150 ul / 10 uM
EUR 145.2

rno-let-7a Primers

MP-r00033 150 ul / 10 uM
EUR 211.2

mmu-let-7a Primers

MPM00007 150 ul / 10 uM
EUR 145.2

hsa-let-7b-3p Primers

MPH02043 150 ul / 10 uM
EUR 145.2

hsa-let-7b-5p Primers

MPH02044 150 ul / 10 uM
EUR 145.2

hsa-let-7c-3p Primers

MPH02045 150 ul / 10 uM
EUR 145.2

hsa-let-7c-5p Primers

MPH02046 150 ul / 10 uM
EUR 145.2

hsa-let-7d-3p Primers

MPH02047 150 ul / 10 uM
EUR 145.2

hsa-let-7d-5p Primers

MPH02048 150 ul / 10 uM
EUR 145.2

hsa-let-7e-3p Primers

MPH02049 150 ul / 10 uM
EUR 145.2

hsa-let-7e-5p Primers

MPH02050 150 ul / 10 uM
EUR 145.2

hsa-let-7f-5p Primers

MPH02053 150 ul / 10 uM
EUR 145.2

hsa-let-7g-3p Primers

MPH02054 150 ul / 10 uM
EUR 145.2

hsa-let-7g-5p Primers

MPH02055 150 ul / 10 uM
EUR 145.2

hsa-let-7i-3p Primers

MPH02056 150 ul / 10 uM
EUR 145.2

hsa-let-7i-5p Primers

MPH02057 150 ul / 10 uM
EUR 145.2

rno-let-7a-5p Primers

MPR00307 150 ul / 10 uM
EUR 211.2

mmu-let-7a-5p Primers

MPM00736 150 ul / 10 uM
EUR 145.2

hsa-let-7f-1-3p Primers

MPH02051 150 ul / 10 uM
EUR 145.2

hsa-let-7f-2-3p Primers

MPH02052 150 ul / 10 uM
EUR 145.2

rno-let-7a-2-3p Primers

MPR00306 150 ul / 10 uM
EUR 211.2

rno-let-7a-1-3p Primers

MPR00878 150 ul / 10 uM
EUR 211.2

mmu-let-7a-1-3p Primers

MPM00734 150 ul / 10 uM
EUR 145.2

mmu-let-7a-2-3p Primers

MPM00735 150 ul / 10 uM
EUR 145.2

hsa-let-7a RT-PCR Primer Set

abx096769-1nmol 1 nmol
EUR 343.2

rno-let-7d Primers

MP-r00007 150 ul / 10 uM
EUR 211.2

rno-let-7b Primers

MP-r00034 150 ul / 10 uM
EUR 211.2

rno-let-7c Primers

MP-r00035 150 ul / 10 uM
EUR 211.2

rno-let-7e Primers

MP-r00036 150 ul / 10 uM
EUR 211.2

rno-let-7f Primers

MP-r00037 150 ul / 10 uM
EUR 211.2

rno-let-7i Primers

MP-r00038 150 ul / 10 uM
EUR 211.2

mmu-let-7b Primers

MPM00008 150 ul / 10 uM
EUR 145.2

mmu-let-7c Primers

MPM00009 150 ul / 10 uM
EUR 145.2

mmu-let-7d Primers

MPM00010 150 ul / 10 uM
EUR 145.2

mmu-let-7e Primers

MPM00011 150 ul / 10 uM
EUR 145.2

mmu-let-7f Primers

MPM00012 150 ul / 10 uM
EUR 145.2

mmu-let-7g Primers

MPM00013 150 ul / 10 uM
EUR 145.2

mmu-let-7i Primers

MPM00014 150 ul / 10 uM
EUR 145.2

mmu-let-7j Primers

MPM00753 150 ul / 10 uM
EUR 145.2

mmu-let-7k Primers

MPM00754 150 ul / 10 uM
EUR 145.2

rno-miR-7a Primers

MP-r00026 150 ul / 10 uM
EUR 211.2

rno-miR-7a Primers

MP-r00027 150 ul / 10 uM
EUR 211.2

mmu-miR-7a Primers

MPM00701 150 ul / 10 uM
EUR 145.2

hsa-miR-1 Primers

MPH01014 150 ul / 10 uM
EUR 145.2

hsa-miR-7 Primers

MPH01976 150 ul / 10 uM
EUR 145.2

hsa-miR-9 Primers

MPH02015 150 ul / 10 uM
EUR 145.2

rno-let-7b-3p Primers

MPR00308 150 ul / 10 uM
EUR 211.2

rno-let-7b-5p Primers

MPR00309 150 ul / 10 uM
EUR 211.2

rno-let-7c-5p Primers

MPR00312 150 ul / 10 uM
EUR 211.2

rno-let-7d-3p Primers

MPR00313 150 ul / 10 uM
EUR 211.2

rno-let-7d-5p Primers

MPR00314 150 ul / 10 uM
EUR 211.2

rno-let-7e-3p Primers

MPR00315 150 ul / 10 uM
EUR 211.2

rno-let-7e-5p Primers

MPR00316 150 ul / 10 uM
EUR 211.2

rno-let-7f-5p Primers

MPR00319 150 ul / 10 uM
EUR 211.2

rno-let-7g-3p Primers

MPR00320 150 ul / 10 uM
EUR 211.2

rno-let-7g-5p Primers

MPR00321 150 ul / 10 uM
EUR 211.2

rno-let-7i-3p Primers

MPR00322 150 ul / 10 uM
EUR 211.2

rno-let-7i-5p Primers

MPR00323 150 ul / 10 uM
EUR 211.2

mmu-let-7b-3p Primers

MPM00737 150 ul / 10 uM
EUR 145.2

mmu-let-7b-5p Primers

MPM00738 150 ul / 10 uM
EUR 145.2

mmu-let-7c-5p Primers

MPM00741 150 ul / 10 uM
EUR 145.2

mmu-let-7d-3p Primers

MPM00742 150 ul / 10 uM
EUR 145.2

mmu-let-7d-5p Primers

MPM00743 150 ul / 10 uM
EUR 145.2

mmu-let-7e-3p Primers

MPM00744 150 ul / 10 uM
EUR 145.2

mmu-let-7e-5p Primers

MPM00745 150 ul / 10 uM
EUR 145.2

mmu-let-7f-5p Primers

MPM00748 150 ul / 10 uM
EUR 145.2

mmu-let-7g-3p Primers

MPM00749 150 ul / 10 uM
EUR 145.2

mmu-let-7g-5p Primers

MPM00750 150 ul / 10 uM
EUR 145.2

mmu-let-7i-3p Primers

MPM00751 150 ul / 10 uM
EUR 145.2

mmu-let-7i-5p Primers

MPM00752 150 ul / 10 uM
EUR 145.2

hsa-miR-16 Primers

MPH01191 150 ul / 10 uM
EUR 145.2

hsa-miR-17 Primers

MPH01192 150 ul / 10 uM
EUR 145.2

hsa-miR-21 Primers

MPH01259 150 ul / 10 uM
EUR 145.2

hsa-miR-22 Primers

MPH01277 150 ul / 10 uM
EUR 145.2

hsa-miR-24 Primers

MPH01294 150 ul / 10 uM
EUR 145.2

hsa-miR-25 Primers

MPH01295 150 ul / 10 uM
EUR 145.2

hsa-miR-31 Primers

MPH01329 150 ul / 10 uM
EUR 145.2

hsa-miR-32 Primers

MPH01417 150 ul / 10 uM
EUR 145.2

hsa-miR-93 Primers

MPH02022 150 ul / 10 uM
EUR 145.2

hsa-miR-95 Primers

MPH02035 150 ul / 10 uM
EUR 145.2

hsa-miR-96 Primers

MPH02036 150 ul / 10 uM
EUR 145.2

hsa-miR-98 Primers

MPH02037 150 ul / 10 uM
EUR 145.2

hsa-miR-100 Primers

MPH01015 150 ul / 10 uM
EUR 145.2

hsa-miR-101 Primers

MPH01016 150 ul / 10 uM
EUR 145.2

hsa-miR-103 Primers

MPH01017 150 ul / 10 uM
EUR 145.2

hsa-miR-105 Primers

MPH01019 150 ul / 10 uM
EUR 145.2

hsa-miR-107 Primers

MPH01022 150 ul / 10 uM
EUR 145.2

hsa-miR-10a Primers

MPH01023 150 ul / 10 uM
EUR 145.2

hsa-miR-10b Primers

MPH01024 150 ul / 10 uM
EUR 145.2

hsa-miR-122 Primers

MPH01045 150 ul / 10 uM
EUR 145.2

hsa-miR-124 Primers

MPH01060 150 ul / 10 uM
EUR 145.2

hsa-miR-126 Primers

MPH01082 150 ul / 10 uM
EUR 145.2

hsa-miR-128 Primers

MPH01110 150 ul / 10 uM
EUR 145.2

hsa-miR-132 Primers

MPH01144 150 ul / 10 uM
EUR 145.2

hsa-miR-134 Primers

MPH01151 150 ul / 10 uM
EUR 145.2

hsa-miR-136 Primers

MPH01154 150 ul / 10 uM
EUR 145.2

hsa-miR-137 Primers

MPH01155 150 ul / 10 uM
EUR 145.2

hsa-miR-138 Primers

MPH01156 150 ul / 10 uM
EUR 145.2

hsa-miR-141 Primers

MPH01161 150 ul / 10 uM
EUR 145.2

hsa-miR-143 Primers

MPH01164 150 ul / 10 uM
EUR 145.2

hsa-miR-144 Primers

MPH01165 150 ul / 10 uM
EUR 145.2

hsa-miR-145 Primers

MPH01166 150 ul / 10 uM
EUR 145.2

hsa-miR-147 Primers

MPH01172 150 ul / 10 uM
EUR 145.2

hsa-miR-149 Primers

MPH01178 150 ul / 10 uM
EUR 145.2

hsa-miR-150 Primers

MPH01179 150 ul / 10 uM
EUR 145.2

hsa-miR-152 Primers

MPH01182 150 ul / 10 uM
EUR 145.2

hsa-miR-153 Primers

MPH01183 150 ul / 10 uM
EUR 145.2

hsa-miR-154 Primers

MPH01187 150 ul / 10 uM
EUR 145.2

hsa-miR-155 Primers

MPH01188 150 ul / 10 uM
EUR 145.2

hsa-miR-15a Primers

MPH01189 150 ul / 10 uM
EUR 145.2

hsa-miR-15b Primers

MPH01190 150 ul / 10 uM
EUR 145.2

hsa-miR-182 Primers

MPH01197 150 ul / 10 uM
EUR 145.2

hsa-miR-183 Primers

MPH01201 150 ul / 10 uM
EUR 145.2

hsa-miR-184 Primers

MPH01202 150 ul / 10 uM
EUR 145.2

hsa-miR-185 Primers

MPH01203 150 ul / 10 uM
EUR 145.2

hsa-miR-186 Primers

MPH01204 150 ul / 10 uM
EUR 145.2

hsa-miR-187 Primers

MPH01205 150 ul / 10 uM
EUR 145.2

hsa-miR-18a Primers

MPH01208 150 ul / 10 uM
EUR 145.2

hsa-miR-18b Primers

MPH01209 150 ul / 10 uM
EUR 145.2

hsa-miR-190 Primers

MPH01210 150 ul / 10 uM
EUR 145.2

hsa-miR-191 Primers

MPH01214 150 ul / 10 uM
EUR 145.2

hsa-miR-192 Primers

MPH01221 150 ul / 10 uM
EUR 145.2

hsa-miR-194 Primers

MPH01225 150 ul / 10 uM
EUR 145.2

hsa-miR-195 Primers

MPH01226 150 ul / 10 uM
EUR 145.2

hsa-miR-197 Primers

MPH01229 150 ul / 10 uM
EUR 145.2

hsa-miR-198 Primers

MPH01238 150 ul / 10 uM
EUR 145.2

hsa-miR-19a Primers

MPH01242 150 ul / 10 uM
EUR 145.2

hsa-miR-19b Primers

MPH01243 150 ul / 10 uM
EUR 145.2

hsa-miR-202 Primers

MPH01247 150 ul / 10 uM
EUR 145.2

hsa-miR-203 Primers

MPH01248 150 ul / 10 uM
EUR 145.2

hsa-miR-204 Primers

MPH01249 150 ul / 10 uM
EUR 145.2

hsa-miR-205 Primers

MPH01250 150 ul / 10 uM
EUR 145.2

hsa-miR-206 Primers

MPH01254 150 ul / 10 uM
EUR 145.2

hsa-miR-20a Primers

MPH01257 150 ul / 10 uM
EUR 145.2

hsa-miR-20b Primers

MPH01258 150 ul / 10 uM
EUR 145.2

hsa-miR-210 Primers

MPH01260 150 ul / 10 uM
EUR 145.2

hsa-miR-211 Primers

MPH01261 150 ul / 10 uM
EUR 145.2

hsa-miR-212 Primers

MPH01268 150 ul / 10 uM
EUR 145.2

hsa-miR-214 Primers

MPH01269 150 ul / 10 uM
EUR 145.2

hsa-miR-215 Primers

MPH01270 150 ul / 10 uM
EUR 145.2

hsa-miR-218 Primers

MPH01273 150 ul / 10 uM
EUR 145.2

hsa-miR-221 Primers

MPH01281 150 ul / 10 uM
EUR 145.2

hsa-miR-222 Primers

MPH01282 150 ul / 10 uM
EUR 145.2

hsa-miR-223 Primers

MPH01283 150 ul / 10 uM
EUR 145.2

hsa-miR-224 Primers

MPH01284 150 ul / 10 uM
EUR 145.2

hsa-miR-23a Primers

MPH01291 150 ul / 10 uM
EUR 145.2

hsa-miR-23b Primers

MPH01292 150 ul / 10 uM
EUR 145.2

hsa-miR-23c Primers

MPH01293 150 ul / 10 uM
EUR 145.2

hsa-miR-26a Primers

MPH01296 150 ul / 10 uM
EUR 145.2

hsa-miR-26b Primers

MPH01297 150 ul / 10 uM
EUR 145.2

hsa-miR-27a Primers

MPH01298 150 ul / 10 uM
EUR 145.2

hsa-miR-27b Primers

MPH01299 150 ul / 10 uM
EUR 145.2

hsa-miR-297 Primers

MPH01306 150 ul / 10 uM
EUR 145.2

hsa-miR-298 Primers

MPH01307 150 ul / 10 uM
EUR 145.2

hsa-miR-29a Primers

MPH01310 150 ul / 10 uM
EUR 145.2

hsa-miR-29b Primers

MPH01311 150 ul / 10 uM
EUR 145.2

hsa-miR-29c Primers

MPH01312 150 ul / 10 uM
EUR 145.2

hsa-miR-300 Primers

MPH01313 150 ul / 10 uM
EUR 145.2

hsa-miR-30a Primers

MPH01324 150 ul / 10 uM
EUR 145.2

hsa-miR-30b Primers

MPH01325 150 ul / 10 uM
EUR 145.2

hsa-miR-30c Primers

MPH01326 150 ul / 10 uM
EUR 145.2

hsa-miR-30d Primers

MPH01327 150 ul / 10 uM
EUR 145.2

hsa-miR-30e Primers

MPH01328 150 ul / 10 uM
EUR 145.2

hsa-miR-325 Primers

MPH01431 150 ul / 10 uM
EUR 145.2

hsa-miR-326 Primers

MPH01432 150 ul / 10 uM
EUR 145.2

hsa-miR-328 Primers

MPH01433 150 ul / 10 uM
EUR 145.2

hsa-miR-329 Primers

MPH01434 150 ul / 10 uM
EUR 145.2

hsa-miR-335 Primers

MPH01439 150 ul / 10 uM
EUR 145.2

hsa-miR-33a Primers

MPH01446 150 ul / 10 uM
EUR 145.2

hsa-miR-33b Primers

MPH01447 150 ul / 10 uM
EUR 145.2

Agents were stained with Giemsa and shows. sequence analysis performed for TUR / SAM / coxiella_1 (MN917207) isolates and the phylogenetic tree was created. This tree, made in accordance with the IS1111 transposon gene, do not form a distinct branch in terms of affiliation host (goats, sheep, tick, human beings) and geographical distribution. As a result, an important zoonotic agent, C. burnetii diagnosed in sheep fetus aborted and infections have proven to have spread among sheep herd in the Black Sea region. Additionally, four separate tick species found in our region and discovered an important host agent for infection.