A previous study based on mitochondrial DNA markers reported Fasciola hepatica in Algeria. However, the exact species identification is still required. In this report, a total of 68 isolates Fasciola, gathered from high-plateau (Bordj-Bou-Arreridj) and steppes (Djelfa) areas of Algeria, identified at the species level by multiplex PCR and PCR-restriction fragment length polymorphism (RFLP) for carboxykinase nuclear phosphoenolpyruvate (PEPCK) and DNA polymerase delta (pold), respectively. Multiplex PCR results contrary to that of PCR-RFLP; However, the next nucleotide sequencing PEPCK clearly showed that all isolates should be classified as F. hepatica. Both markers of mitochondria, NADH dehydrogenase subunit I (nad1) and cytochrome c oxidase subunit 1 (COX1), revealed a close relationship between the parasite populations of the highlands and the people of the savannah. A direction of the spread of highland pasture is indicated as a former resident of the more diversified than the last. In addition, the population is more closely related to the population of Spain than those of Egypt or Afghanistan. Given the characteristics of the population of F. hepatica in Spain and the history of the cattle trade, it seems likely that the parasite was introduced into Algeria from Europe via the Mediterranean sea.We current pandemic of COVID-19, which is caused by spillover from animal-derived human coronavirus occurred at Wuhan region of China in December 2019. China, the virus has spread to 188 countries and territories around the world, reached the Sahel region on March 2, 2020. Since the whole-genome sequencing (WGS) data is very important to understand the dynamics of the spread of the pandemic in progress but only limited data available from the Sahel region sequenced to date, we have focused our efforts on producing the first Mali sequencing data available. Screening Mali 217 patient samples for the presence of SARS-CoV-2 resulted in 38 positive isolates, of which 21 whole genome sequences generated. Our analysis shows that both A beginning (19B) and then observed B (20A / C) clade were present in Mali, showed few and independent introductions of SARS-CoV-2 to the Sahel region.

Molecular phylogenetic and genetic variability Fasciola gigantica in the province of Kermanshah, western Iran with a view to understanding the haplotype distribution in Asia and Africa

Over the past decade, diagnostic tools to detect and differentiate Fasciola species has improved, but our understanding of the haplotype distribution and population structure of the parasite is less clear. This study was designed to survey this gap in F. gigantica epidemiology in Kermanshah province, western Iran from 2015 to 2017. Sixty-eight Fasciola isolates collected from slaughterhouses from the province. We evaluated test PCR-RFLP of ITS1 gene for identification of species Fasciola using enzymes RsaI. After the identification of species Fasciola, partial sequence of mitochondrial NADH dehydrogenase subunit 1 (ND1) gene F. gigantica used for the subsequent development of the tree and phylogenetic analyzes network. PRFLP PCR-based pro fi le, one (6.25%) of the isolates of sheep and 19 (39.60%) of the isolates were detected as F. gigantica livestock, while 93.75% of the isolates sheep, 60.40% of the isolates cattle and goats are all F. hepatica isolates. In 20 worms were analyzed, five ND1 haplotypes were detected. Statistically significant genetic differences shown between the Iranian population and all other populations. Evidence is presented for the existence of two separate populations: African and West Asian Worms Worms gigantica gigantica and East Asia. Genetic relationship between haplotype associated with geographic divisions.

 Molecular characterization and phylogenetic analysis of Fasciola hepatica from high- plateau and steppe areas in Algeria
Molecular characterization and phylogenetic analysis of Fasciola hepatica from high- plateau and steppe areas in Algeria

 

probe molecule, and analsysis phylogenetic isolation of Coxiella burnetii from aborted fetuses and ticks

Q fever is a zoonotic infection threaten human health, causes abortion in cattle, sheep and goats. Coxiella burnetii (C. burnetii) also lead to serious problems such as low birth weight, infertility. This study is the first example for the analysis of Q fever in the area around the Black Sea in Turkey. In the study, 270 of aborted fetuses (171 cows, 79 goats, 20 goats) and 1069 tick samples intended to be sought by PCR. C. burnetii DNA was detected in 8 (2.96%) of 270 specimens of sheep while it was not to be found in cows and goats specimens. 406 sample pool of 1069 created tick samples (490 men, 579 women) were collected from 254 cattle (187 cows, 54 goats, 13 goats) and 11 of them tested positive. Tick ​​species are defined as positive C. burnetii Hyalomma marginatum, Hyalomma anoliticum excavatum, Hyalomma detritum and Boophilus annulatus. Agent isolation done in embryonated eggs.

hsa-let-7a-5p Primers

MPH02042 150 ul / 10 uM
EUR 121

hsa-let-7a-2-3p Primers

MPH03980 150 ul / 10 uM
EUR 121

mmu-let-7a Primers

MPM00007 150 ul / 10 uM
EUR 121

rno-let-7a Primers

MP-r00033 150 ul / 10 uM
EUR 176

Mouse pre-microRNA Expression Construct let-7a-1

MMIR-let-7a-1-PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

Mouse pre-microRNA Expression Construct let-7a-2

MMIR-let-7a-2-PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

mmu-let-7a-5p Primers

MPM00736 150 ul / 10 uM
EUR 121

rno-let-7a-5p Primers

MPR00307 150 ul / 10 uM
EUR 176

hsa-let-7a miRNA Inhibitor

MIH01000 2 x 5.0 nmol
EUR 176

hsa-let-7a miRNA Antagomir

MNH01000 2 x 5.0 nmol
EUR 329

pLL3.7- Hsa- Let- 7a- 1miRNA

PVT10410 2 ug
EUR 301

hsa-let-7b Primers

MPH01007 150 ul / 10 uM
EUR 121

hsa-let-7c Primers

MPH01008 150 ul / 10 uM
EUR 121

hsa-let-7d Primers

MPH01009 150 ul / 10 uM
EUR 121

hsa-let-7e Primers

MPH01010 150 ul / 10 uM
EUR 121

hsa-let-7f Primers

MPH01011 150 ul / 10 uM
EUR 121

hsa-let-7g Primers

MPH01012 150 ul / 10 uM
EUR 121

hsa-let-7i Primers

MPH01013 150 ul / 10 uM
EUR 121

mmu-let-7a-1-3p Primers

MPM00734 150 ul / 10 uM
EUR 121

mmu-let-7a-2-3p Primers

MPM00735 150 ul / 10 uM
EUR 121

rno-let-7a-2-3p Primers

MPR00306 150 ul / 10 uM
EUR 176

rno-let-7a-1-3p Primers

MPR00878 150 ul / 10 uM
EUR 176

hsa-let-7a-3p miRNA Inhibitor

MIH01002 2 x 5.0 nmol
EUR 176

hsa-let-7a-5p miRNA Inhibitor

MIH01003 2 x 5.0 nmol
EUR 176

hsa-let-7a-3p miRNA Antagomir

MNH01002 2 x 5.0 nmol
EUR 329

hsa-let-7a-5p miRNA Antagomir

MNH01003 2 x 5.0 nmol
EUR 329

hsa-let-7b-3p Primers

MPH02043 150 ul / 10 uM
EUR 121

hsa-let-7b-5p Primers

MPH02044 150 ul / 10 uM
EUR 121

hsa-let-7c-3p Primers

MPH02045 150 ul / 10 uM
EUR 121

hsa-let-7c-5p Primers

MPH02046 150 ul / 10 uM
EUR 121

hsa-let-7d-3p Primers

MPH02047 150 ul / 10 uM
EUR 121

hsa-let-7d-5p Primers

MPH02048 150 ul / 10 uM
EUR 121

hsa-let-7e-3p Primers

MPH02049 150 ul / 10 uM
EUR 121

hsa-let-7e-5p Primers

MPH02050 150 ul / 10 uM
EUR 121

hsa-let-7f-5p Primers

MPH02053 150 ul / 10 uM
EUR 121

hsa-let-7g-3p Primers

MPH02054 150 ul / 10 uM
EUR 121

hsa-let-7g-5p Primers

MPH02055 150 ul / 10 uM
EUR 121

hsa-let-7i-3p Primers

MPH02056 150 ul / 10 uM
EUR 121

hsa-let-7i-5p Primers

MPH02057 150 ul / 10 uM
EUR 121

hsa-let-7a RT-PCR Detection Kit

20-abx096015
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

hsa-let-7a RT-PCR Primer Set

abx096769-1nmol 1 nmol
EUR 286
  • Shipped within 5-10 working days.

hsa-let-7a-2-3p miRNA Inhibitor

MIH01001 2 x 5.0 nmol
EUR 176

hsa-let-7a-2-3p miRNA Antagomir

MNH01001 2 x 5.0 nmol
EUR 329

hsa-let-7f-1-3p Primers

MPH02051 150 ul / 10 uM
EUR 121

hsa-let-7f-2-3p Primers

MPH02052 150 ul / 10 uM
EUR 121

rno-let-7a miRNA Antagomir

MNR01000 2 x 5.0 nmol
EUR 329

mmu-let-7a miRNA Inhibitor

MIM01000 2 x 5.0 nmol
EUR 176

rno-let-7a miRNA Inhibitor

MIR01000 2 x 5.0 nmol
EUR 176

mmu-let-7a miRNA Antagomir

MNM01000 2 x 5.0 nmol
EUR 329

hsa-let-7a RT-PCR Detection and U6 Calibration Kit

20-abx096392
  • EUR 690.00
  • EUR 954.00
  • EUR 495.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

rno-let-7a-5p miRNA Antagomir

MNR01003 2 x 5.0 nmol
EUR 329

mmu-let-7a-5p miRNA Inhibitor

MIM01003 2 x 5.0 nmol
EUR 176

rno-let-7a-5p miRNA Inhibitor

MIR01003 2 x 5.0 nmol
EUR 176

mmu-let-7a-5p miRNA Antagomir

MNM01003 2 x 5.0 nmol
EUR 329

mmu-let-7j Primers

MPM00753 150 ul / 10 uM
EUR 121

mmu-let-7k Primers

MPM00754 150 ul / 10 uM
EUR 121

mmu-let-7b Primers

MPM00008 150 ul / 10 uM
EUR 121

mmu-let-7c Primers

MPM00009 150 ul / 10 uM
EUR 121

mmu-let-7d Primers

MPM00010 150 ul / 10 uM
EUR 121

mmu-let-7e Primers

MPM00011 150 ul / 10 uM
EUR 121

mmu-let-7f Primers

MPM00012 150 ul / 10 uM
EUR 121

mmu-let-7g Primers

MPM00013 150 ul / 10 uM
EUR 121

mmu-let-7i Primers

MPM00014 150 ul / 10 uM
EUR 121

rno-let-7d Primers

MP-r00007 150 ul / 10 uM
EUR 176

rno-let-7b Primers

MP-r00034 150 ul / 10 uM
EUR 176

rno-let-7c Primers

MP-r00035 150 ul / 10 uM
EUR 176

rno-let-7e Primers

MP-r00036 150 ul / 10 uM
EUR 176

rno-let-7f Primers

MP-r00037 150 ul / 10 uM
EUR 176

rno-let-7i Primers

MP-r00038 150 ul / 10 uM
EUR 176

mmu-miR-7a Primers

MPM00701 150 ul / 10 uM
EUR 121

rno-miR-7a Primers

MP-r00026 150 ul / 10 uM
EUR 176

rno-miR-7a Primers

MP-r00027 150 ul / 10 uM
EUR 176

rno-let-7a-1-3p miRNA Antagomir

MNR01001 2 x 5.0 nmol
EUR 329

rno-let-7a-2-3p miRNA Antagomir

MNR01002 2 x 5.0 nmol
EUR 329

mmu-let-7a-1-3p miRNA Inhibitor

MIM01001 2 x 5.0 nmol
EUR 176

mmu-let-7a-2-3p miRNA Inhibitor

MIM01002 2 x 5.0 nmol
EUR 176

rno-let-7a-1-3p miRNA Inhibitor

MIR01001 2 x 5.0 nmol
EUR 176

rno-let-7a-2-3p miRNA Inhibitor

MIR01002 2 x 5.0 nmol
EUR 176

mmu-let-7a-1-3p miRNA Antagomir

MNM01001 2 x 5.0 nmol
EUR 329

mmu-let-7a-2-3p miRNA Antagomir

MNM01002 2 x 5.0 nmol
EUR 329

mmu-let-7b-3p Primers

MPM00737 150 ul / 10 uM
EUR 121

mmu-let-7b-5p Primers

MPM00738 150 ul / 10 uM
EUR 121

mmu-let-7c-5p Primers

MPM00741 150 ul / 10 uM
EUR 121

mmu-let-7d-3p Primers

MPM00742 150 ul / 10 uM
EUR 121

mmu-let-7d-5p Primers

MPM00743 150 ul / 10 uM
EUR 121

mmu-let-7e-3p Primers

MPM00744 150 ul / 10 uM
EUR 121

mmu-let-7e-5p Primers

MPM00745 150 ul / 10 uM
EUR 121

mmu-let-7f-5p Primers

MPM00748 150 ul / 10 uM
EUR 121

mmu-let-7g-3p Primers

MPM00749 150 ul / 10 uM
EUR 121

mmu-let-7g-5p Primers

MPM00750 150 ul / 10 uM
EUR 121

mmu-let-7i-3p Primers

MPM00751 150 ul / 10 uM
EUR 121

mmu-let-7i-5p Primers

MPM00752 150 ul / 10 uM
EUR 121

rno-let-7b-3p Primers

MPR00308 150 ul / 10 uM
EUR 176

rno-let-7b-5p Primers

MPR00309 150 ul / 10 uM
EUR 176

rno-let-7c-5p Primers

MPR00312 150 ul / 10 uM
EUR 176

rno-let-7d-3p Primers

MPR00313 150 ul / 10 uM
EUR 176

rno-let-7d-5p Primers

MPR00314 150 ul / 10 uM
EUR 176

rno-let-7e-3p Primers

MPR00315 150 ul / 10 uM
EUR 176

rno-let-7e-5p Primers

MPR00316 150 ul / 10 uM
EUR 176

rno-let-7f-5p Primers

MPR00319 150 ul / 10 uM
EUR 176

rno-let-7g-3p Primers

MPR00320 150 ul / 10 uM
EUR 176

rno-let-7g-5p Primers

MPR00321 150 ul / 10 uM
EUR 176

rno-let-7i-3p Primers

MPR00322 150 ul / 10 uM
EUR 176

rno-let-7i-5p Primers

MPR00323 150 ul / 10 uM
EUR 176

Dinitrophenyl (DNP)-HSA protein Conjugate

AV-9330-HSA 10 mg
EUR 286

hsa-let-7b miRNA Inhibitor

MIH01004 2 x 5.0 nmol
EUR 176

hsa-let-7c miRNA Inhibitor

MIH01007 2 x 5.0 nmol
EUR 176

hsa-let-7d miRNA Inhibitor

MIH01010 2 x 5.0 nmol
EUR 176

hsa-let-7e miRNA Inhibitor

MIH01013 2 x 5.0 nmol
EUR 176

hsa-let-7g miRNA Inhibitor

MIH01020 2 x 5.0 nmol
EUR 176

hsa-let-7i miRNA Inhibitor

MIH01023 2 x 5.0 nmol
EUR 176

hsa-let-7b miRNA Antagomir

MNH01004 2 x 5.0 nmol
EUR 329

hsa-let-7c miRNA Antagomir

MNH01007 2 x 5.0 nmol
EUR 329

hsa-let-7d miRNA Antagomir

MNH01010 2 x 5.0 nmol
EUR 329

hsa-let-7e miRNA Antagomir

MNH01013 2 x 5.0 nmol
EUR 329

hsa-let-7f miRNA Antagomir

MNH01016 2 x 5.0 nmol
EUR 329

hsa-let-7g miRNA Antagomir

MNH01020 2 x 5.0 nmol
EUR 329

hsa-let-7i miRNA Antagomir

MNH01023 2 x 5.0 nmol
EUR 329

mmu-miR-7a-5p Primers

MPM02196 150 ul / 10 uM
EUR 121

rno-miR-7a-5p Primers

MPR00838 150 ul / 10 uM
EUR 176

rno-let-7a-1* RT-PCR Detection Kit

20-abx096331
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

rno-let-7a-1* RT-PCR Primer Set

abx097085-1nmol 1 nmol
EUR 286
  • Shipped within 5-10 working days.

Human pre-microRNA Expression Construct let-7a-1

PMIRHlet7a1PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

Human pre-microRNA Expression Construct let-7a-2

PMIRHlet7a2PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

Human pre-microRNA Expression Construct let-7a-3

PMIRHlet7a3PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

mmu-let-7c-1-3p Primers

MPM00739 150 ul / 10 uM
EUR 121

mmu-let-7c-2-3p Primers

MPM00740 150 ul / 10 uM
EUR 121

mmu-let-7f-1-3p Primers

MPM00746 150 ul / 10 uM
EUR 121

mmu-let-7f-2-3p Primers

MPM00747 150 ul / 10 uM
EUR 121

rno-let-7c-1-3p Primers

MPR00310 150 ul / 10 uM
EUR 176

rno-let-7c-2-3p Primers

MPR00311 150 ul / 10 uM
EUR 176

rno-let-7f-1-3p Primers

MPR00317 150 ul / 10 uM
EUR 176

rno-let-7f-2-3p Primers

MPR00318 150 ul / 10 uM
EUR 176

hsa-let-7a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097603
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-let-7b-3p miRNA Inhibitor

MIH01005 2 x 5.0 nmol
EUR 176

hsa-let-7b-5p miRNA Inhibitor

MIH01006 2 x 5.0 nmol
EUR 176

hsa-let-7c-3p miRNA Inhibitor

MIH01008 2 x 5.0 nmol
EUR 176

hsa-let-7c-5p miRNA Inhibitor

MIH01009 2 x 5.0 nmol
EUR 176

hsa-let-7d-3p miRNA Inhibitor

MIH01011 2 x 5.0 nmol
EUR 176

hsa-let-7d-5p miRNA Inhibitor

MIH01012 2 x 5.0 nmol
EUR 176

hsa-let-7e-3p miRNA Inhibitor

MIH01014 2 x 5.0 nmol
EUR 176

hsa-let-7e-5p miRNA Inhibitor

MIH01015 2 x 5.0 nmol
EUR 176

hsa-let-7f-5p miRNA Inhibitor

MIH01019 2 x 5.0 nmol
EUR 176

hsa-let-7g-3p miRNA Inhibitor

MIH01021 2 x 5.0 nmol
EUR 176

hsa-let-7g-5p miRNA Inhibitor

MIH01022 2 x 5.0 nmol
EUR 176

hsa-let-7i-3p miRNA Inhibitor

MIH01024 2 x 5.0 nmol
EUR 176

hsa-let-7i-5p miRNA Inhibitor

MIH01025 2 x 5.0 nmol
EUR 176

hsa-let-7b-3p miRNA Antagomir

MNH01005 2 x 5.0 nmol
EUR 329

hsa-let-7b-5p miRNA Antagomir

MNH01006 2 x 5.0 nmol
EUR 329

hsa-let-7c-3p miRNA Antagomir

MNH01008 2 x 5.0 nmol
EUR 329

hsa-let-7c-5p miRNA Antagomir

MNH01009 2 x 5.0 nmol
EUR 329

hsa-let-7d-3p miRNA Antagomir

MNH01011 2 x 5.0 nmol
EUR 329

hsa-let-7d-5p miRNA Antagomir

MNH01012 2 x 5.0 nmol
EUR 329

hsa-let-7e-3p miRNA Antagomir

MNH01014 2 x 5.0 nmol
EUR 329

hsa-let-7e-5p miRNA Antagomir

MNH01015 2 x 5.0 nmol
EUR 329

hsa-let-7f-5p miRNA Antagomir

MNH01019 2 x 5.0 nmol
EUR 329

hsa-let-7g-3p miRNA Antagomir

MNH01021 2 x 5.0 nmol
EUR 329

hsa-let-7g-5p miRNA Antagomir

MNH01022 2 x 5.0 nmol
EUR 329

hsa-let-7i-3p miRNA Antagomir

MNH01024 2 x 5.0 nmol
EUR 329

hsa-let-7i-5p miRNA Antagomir

MNH01025 2 x 5.0 nmol
EUR 329

mmu-miR-7a-1-3p Primers

MPM02194 150 ul / 10 uM
EUR 121

mmu-miR-7a-2-3p Primers

MPM02195 150 ul / 10 uM
EUR 121

rno-miR-7a-1-3p Primers

MPR00836 150 ul / 10 uM
EUR 176

rno-miR-7a-2-3p Primers

MPR00837 150 ul / 10 uM
EUR 176

Mouse pre-microRNA Expression Construct let-7b

MMIR-let-7b-PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

Mouse pre-microRNA Expression Construct let-7d

MMIR-let-7d-PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

Mouse pre-microRNA Expression Construct let-7e

MMIR-let-7e-PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

Mouse pre-microRNA Expression Construct let-7g

MMIR-let-7g-PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

Mouse pre-microRNA Expression Construct mir-7a-1

MMIR-7a-1-PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

Mouse pre-microRNA Expression Construct mir-7a-2

MMIR-7a-2-PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

hsa-let-7b RT-PCR Detection Kit

20-abx096016
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

hsa-let-7c RT-PCR Detection Kit

20-abx096017
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

hsa-let-7d RT-PCR Detection Kit

20-abx096018
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

hsa-let-7e RT-PCR Detection Kit

20-abx096019
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

hsa-let-7f RT-PCR Detection Kit

20-abx096020
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

hsa-let-7g RT-PCR Detection Kit

20-abx096021
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

Agents were stained with Giemsa and shows. sequence analysis performed for TUR / SAM / coxiella_1 (MN917207) isolates and the phylogenetic tree was created. This tree, made in accordance with the IS1111 transposon gene, do not form a distinct branch in terms of affiliation host (goats, sheep, tick, human beings) and geographical distribution. As a result, an important zoonotic agent, C. burnetii diagnosed in sheep fetus aborted and infections have proven to have spread among sheep herd in the Black Sea region. Additionally, four separate tick species found in our region and discovered an important host agent for infection.