Molecular phylogenetic position of Minamitalitrus zoltani elucidates a further troglobisation pattern in cave-dwelling terrestrial amphipods (Crustacea: Talitridae)

Talitrids is a very diverse group of amphipod crustaceans that have been colonized by a variety of terrestrial habitats. Three genera have been successfully adapted to cave habitats on islands in the Pacific and Atlantic Oceans. However, the evolutionary origin of the Pacific talitrids troglobitic remain unknown. We estimate the phylogenetic position of Minamitalitrus zoltani troglobitic, which inhabits limestone cave in Minamidaito Island in the Northwestern Pacific, on the basis of the traditional multi-locus datasets. For talitrids analyzed, we also reconstruct the ancestral homeland of palp maxilliped and gnathopod male 2.

Our results show that Minamitalitrus zoltani is sister to curvatus Nipponorchestia epigean with profound differences. Nipponorchestia curvatus inhabit coastal habitats in Japan, but it is not customary for Minamidaito Island. Previous research estimates that the Atlantic troglobitic species have invaded the underground habitat several times, but we provide new insights into the history troglobisation in talitrids. We also recover secondary shift state palp character maxilliped and men gnathopod 2 in the lineage consists of Minamitalitrus and genera phylogenetically closely.

Our findings highlight the need for reclassification genus-the genus level; Nipponorchestia we split into two genera, establish a new genus for Nipponorchestia nudiramus. A phylogenetic analysis of genera of strongyloid Cloacininae sub-family of marsupials macropodoid in Australasia is based on morphological characteristics and sequence analysis continued (+ ITS) first (ITS-1) and second (ITS-2) internal transcribed spacer of ribosomal DNA. Both approaches are provided robust phylogeny, but the similarities between the two methods in terms of generic grouping suggests that needed substantial revision of the classification phenetic this time, with some morphological characteristics of the current key used for defining genera and tribes prove to be homoplasious.

Molecular phylogenetic, population genetic and demographic studies and Nodularia Nodularia douglasiae breviconcha based CO1 and 16S rRNA

freshwater mussels belonging to the genus Nodularia (Family Unionidae) is known to be widely distributed in East Asia. Although phylogenetic and population genetic studies have been conducted on this species, remains unresolved questions in the taxonomic status and biogeographic distribution channels. Here, the nucleotide sequence of 16S rRNA CO1 and a newly defined than 86 N. douglasiae and 83 individuals N. breviconcha collected on the Korean Peninsula.

Based on these data, we revealed the following results: (1) N. douglasiae can be divided into three genetic clades A (found only on the Korean Peninsula), B (widely distributed in East Asia), and C (found only in western China and Russian), (2) a clade A is not an independent species but concrete member N. douglasiae given the lack of genetic differences between clades A and B, and (3) N. breviconcha not subspecies N. douglasiae but independent species apart from N. douglasiae. In addition, we suggest plausible scenario events biogeographic distribution and demographic history Nodularia species.

 Molecular phylogenetic position of Minamitalitrus zoltani elucidates a further troglobisation pattern in cave-dwelling terrestrial amphipods (Crustacea: Talitridae)
Molecular phylogenetic position of Minamitalitrus zoltani elucidates a further troglobisation pattern in cave-dwelling terrestrial amphipods (Crustacea: Talitridae)

molecular phylogenetics of Lotus (Leguminosae) with an emphasis in tempo and pattern of colonization in the region Macaronesian

With a wide variety of distribution including Europe and Asia, Lotus (Leguminosae) is the largest genus in Loteae. This is especially diverse in the Mediterreanean and the five islands of Macaronesia (the Atlantic Ocean). However, little is known about the relationship between section 14 currently recognized in the Lotus and about the timing and pattern of colonization in the Macaronesian region. In this study, we used the four regions of DNA (ribosomal nuclear ITS plus three regions plastid) in sampling comprehensively most species Lotus to date (some endemic species in the Canary Islands which are under-represented in the phylogenetic analysis before) to infer relationships within this genus and for establishing patterns of colonization in Macaronesia.

Divergence time estimates and habitat reconstruction analysis showed that Lotus possibility diverged about 7.86 Ma of the sister group, but all the events of colonization to occur Macaronesia recently (starting from the latter 0.23 to 2.70 Ma). Diversification Lotus in Macaronesia involved between four and six independent colonization events of the four sections is currently distributed in Africa and Europe.

hsa-let-7e Primers

MPH01010 150 ul / 10 uM
EUR 145.2

hsa-let-7f Primers

MPH01011 150 ul / 10 uM
EUR 145.2

hsa-let-7g Primers

MPH01012 150 ul / 10 uM
EUR 145.2

hsa-let-7i Primers

MPH01013 150 ul / 10 uM
EUR 145.2

rno-let-7c-5p Primers

MPR00312 150 ul / 10 uM
EUR 211.2

mmu-let-7c-5p Primers

MPM00741 150 ul / 10 uM
EUR 145.2

hsa-let-7a-3p Primers

MPH02041 150 ul / 10 uM
EUR 145.2

hsa-let-7a-5p Primers

MPH02042 150 ul / 10 uM
EUR 145.2

hsa-let-7b-3p Primers

MPH02043 150 ul / 10 uM
EUR 145.2

hsa-let-7b-5p Primers

MPH02044 150 ul / 10 uM
EUR 145.2

hsa-let-7d-3p Primers

MPH02047 150 ul / 10 uM
EUR 145.2

hsa-let-7d-5p Primers

MPH02048 150 ul / 10 uM
EUR 145.2

hsa-let-7e-3p Primers

MPH02049 150 ul / 10 uM
EUR 145.2

hsa-let-7e-5p Primers

MPH02050 150 ul / 10 uM
EUR 145.2

hsa-let-7f-5p Primers

MPH02053 150 ul / 10 uM
EUR 145.2

hsa-let-7g-3p Primers

MPH02054 150 ul / 10 uM
EUR 145.2

hsa-let-7g-5p Primers

MPH02055 150 ul / 10 uM
EUR 145.2

hsa-let-7i-3p Primers

MPH02056 150 ul / 10 uM
EUR 145.2

hsa-let-7i-5p Primers

MPH02057 150 ul / 10 uM
EUR 145.2

rno-let-7c-1-3p Primers

MPR00310 150 ul / 10 uM
EUR 211.2

rno-let-7c-2-3p Primers

MPR00311 150 ul / 10 uM
EUR 211.2

mmu-let-7c-1-3p Primers

MPM00739 150 ul / 10 uM
EUR 145.2

mmu-let-7c-2-3p Primers

MPM00740 150 ul / 10 uM
EUR 145.2

hsa-let-7f-1-3p Primers

MPH02051 150 ul / 10 uM
EUR 145.2

hsa-let-7f-2-3p Primers

MPH02052 150 ul / 10 uM
EUR 145.2

hsa-let-7a-2-3p Primers

MPH03980 150 ul / 10 uM
EUR 145.2

hsa-let-7c RT-PCR Primer Set

abx096771-1nmol 1 nmol
EUR 343.2

rno-let-7d Primers

MP-r00007 150 ul / 10 uM
EUR 211.2

rno-let-7a Primers

MP-r00033 150 ul / 10 uM
EUR 211.2

rno-let-7b Primers

MP-r00034 150 ul / 10 uM
EUR 211.2

rno-let-7e Primers

MP-r00036 150 ul / 10 uM
EUR 211.2

rno-let-7f Primers

MP-r00037 150 ul / 10 uM
EUR 211.2

rno-let-7i Primers

MP-r00038 150 ul / 10 uM
EUR 211.2

mmu-let-7a Primers

MPM00007 150 ul / 10 uM
EUR 145.2

mmu-let-7b Primers

MPM00008 150 ul / 10 uM
EUR 145.2

mmu-let-7d Primers

MPM00010 150 ul / 10 uM
EUR 145.2

mmu-let-7e Primers

MPM00011 150 ul / 10 uM
EUR 145.2

mmu-let-7f Primers

MPM00012 150 ul / 10 uM
EUR 145.2

mmu-let-7g Primers

MPM00013 150 ul / 10 uM
EUR 145.2

mmu-let-7i Primers

MPM00014 150 ul / 10 uM
EUR 145.2

mmu-let-7j Primers

MPM00753 150 ul / 10 uM
EUR 145.2

mmu-let-7k Primers

MPM00754 150 ul / 10 uM
EUR 145.2

hsa-miR-1 Primers

MPH01014 150 ul / 10 uM
EUR 145.2

hsa-miR-7 Primers

MPH01976 150 ul / 10 uM
EUR 145.2

hsa-miR-9 Primers

MPH02015 150 ul / 10 uM
EUR 145.2

hsa-miR-16 Primers

MPH01191 150 ul / 10 uM
EUR 145.2

hsa-miR-17 Primers

MPH01192 150 ul / 10 uM
EUR 145.2

hsa-miR-21 Primers

MPH01259 150 ul / 10 uM
EUR 145.2

hsa-miR-22 Primers

MPH01277 150 ul / 10 uM
EUR 145.2

hsa-miR-24 Primers

MPH01294 150 ul / 10 uM
EUR 145.2

hsa-miR-25 Primers

MPH01295 150 ul / 10 uM
EUR 145.2

hsa-miR-31 Primers

MPH01329 150 ul / 10 uM
EUR 145.2

hsa-miR-32 Primers

MPH01417 150 ul / 10 uM
EUR 145.2

hsa-miR-93 Primers

MPH02022 150 ul / 10 uM
EUR 145.2

hsa-miR-95 Primers

MPH02035 150 ul / 10 uM
EUR 145.2

hsa-miR-96 Primers

MPH02036 150 ul / 10 uM
EUR 145.2

hsa-miR-98 Primers

MPH02037 150 ul / 10 uM
EUR 145.2

rno-let-7a-5p Primers

MPR00307 150 ul / 10 uM
EUR 211.2

rno-let-7b-3p Primers

MPR00308 150 ul / 10 uM
EUR 211.2

rno-let-7b-5p Primers

MPR00309 150 ul / 10 uM
EUR 211.2

rno-let-7d-3p Primers

MPR00313 150 ul / 10 uM
EUR 211.2

rno-let-7d-5p Primers

MPR00314 150 ul / 10 uM
EUR 211.2

rno-let-7e-3p Primers

MPR00315 150 ul / 10 uM
EUR 211.2

rno-let-7e-5p Primers

MPR00316 150 ul / 10 uM
EUR 211.2

rno-let-7f-5p Primers

MPR00319 150 ul / 10 uM
EUR 211.2

rno-let-7g-3p Primers

MPR00320 150 ul / 10 uM
EUR 211.2

rno-let-7g-5p Primers

MPR00321 150 ul / 10 uM
EUR 211.2

rno-let-7i-3p Primers

MPR00322 150 ul / 10 uM
EUR 211.2

rno-let-7i-5p Primers

MPR00323 150 ul / 10 uM
EUR 211.2

mmu-let-7a-5p Primers

MPM00736 150 ul / 10 uM
EUR 145.2

mmu-let-7b-3p Primers

MPM00737 150 ul / 10 uM
EUR 145.2

mmu-let-7b-5p Primers

MPM00738 150 ul / 10 uM
EUR 145.2

mmu-let-7d-3p Primers

MPM00742 150 ul / 10 uM
EUR 145.2

mmu-let-7d-5p Primers

MPM00743 150 ul / 10 uM
EUR 145.2

mmu-let-7e-3p Primers

MPM00744 150 ul / 10 uM
EUR 145.2

mmu-let-7e-5p Primers

MPM00745 150 ul / 10 uM
EUR 145.2

mmu-let-7f-5p Primers

MPM00748 150 ul / 10 uM
EUR 145.2

mmu-let-7g-3p Primers

MPM00749 150 ul / 10 uM
EUR 145.2

mmu-let-7g-5p Primers

MPM00750 150 ul / 10 uM
EUR 145.2

mmu-let-7i-3p Primers

MPM00751 150 ul / 10 uM
EUR 145.2

mmu-let-7i-5p Primers

MPM00752 150 ul / 10 uM
EUR 145.2

hsa-miR-100 Primers

MPH01015 150 ul / 10 uM
EUR 145.2

hsa-miR-101 Primers

MPH01016 150 ul / 10 uM
EUR 145.2

hsa-miR-103 Primers

MPH01017 150 ul / 10 uM
EUR 145.2

hsa-miR-105 Primers

MPH01019 150 ul / 10 uM
EUR 145.2

hsa-miR-107 Primers

MPH01022 150 ul / 10 uM
EUR 145.2

hsa-miR-10a Primers

MPH01023 150 ul / 10 uM
EUR 145.2

hsa-miR-10b Primers

MPH01024 150 ul / 10 uM
EUR 145.2

hsa-miR-122 Primers

MPH01045 150 ul / 10 uM
EUR 145.2

hsa-miR-124 Primers

MPH01060 150 ul / 10 uM
EUR 145.2

hsa-miR-126 Primers

MPH01082 150 ul / 10 uM
EUR 145.2

hsa-miR-128 Primers

MPH01110 150 ul / 10 uM
EUR 145.2

hsa-miR-132 Primers

MPH01144 150 ul / 10 uM
EUR 145.2

hsa-miR-134 Primers

MPH01151 150 ul / 10 uM
EUR 145.2

hsa-miR-136 Primers

MPH01154 150 ul / 10 uM
EUR 145.2

hsa-miR-137 Primers

MPH01155 150 ul / 10 uM
EUR 145.2

hsa-miR-138 Primers

MPH01156 150 ul / 10 uM
EUR 145.2

hsa-miR-141 Primers

MPH01161 150 ul / 10 uM
EUR 145.2

hsa-miR-143 Primers

MPH01164 150 ul / 10 uM
EUR 145.2

hsa-miR-144 Primers

MPH01165 150 ul / 10 uM
EUR 145.2

hsa-miR-145 Primers

MPH01166 150 ul / 10 uM
EUR 145.2

hsa-miR-147 Primers

MPH01172 150 ul / 10 uM
EUR 145.2

hsa-miR-149 Primers

MPH01178 150 ul / 10 uM
EUR 145.2

hsa-miR-150 Primers

MPH01179 150 ul / 10 uM
EUR 145.2

hsa-miR-152 Primers

MPH01182 150 ul / 10 uM
EUR 145.2

hsa-miR-153 Primers

MPH01183 150 ul / 10 uM
EUR 145.2

hsa-miR-154 Primers

MPH01187 150 ul / 10 uM
EUR 145.2

hsa-miR-155 Primers

MPH01188 150 ul / 10 uM
EUR 145.2

hsa-miR-15a Primers

MPH01189 150 ul / 10 uM
EUR 145.2

hsa-miR-15b Primers

MPH01190 150 ul / 10 uM
EUR 145.2

hsa-miR-182 Primers

MPH01197 150 ul / 10 uM
EUR 145.2

hsa-miR-183 Primers

MPH01201 150 ul / 10 uM
EUR 145.2

hsa-miR-184 Primers

MPH01202 150 ul / 10 uM
EUR 145.2

hsa-miR-185 Primers

MPH01203 150 ul / 10 uM
EUR 145.2

hsa-miR-186 Primers

MPH01204 150 ul / 10 uM
EUR 145.2

hsa-miR-187 Primers

MPH01205 150 ul / 10 uM
EUR 145.2

hsa-miR-18a Primers

MPH01208 150 ul / 10 uM
EUR 145.2

hsa-miR-18b Primers

MPH01209 150 ul / 10 uM
EUR 145.2

hsa-miR-190 Primers

MPH01210 150 ul / 10 uM
EUR 145.2

hsa-miR-191 Primers

MPH01214 150 ul / 10 uM
EUR 145.2

hsa-miR-192 Primers

MPH01221 150 ul / 10 uM
EUR 145.2

hsa-miR-194 Primers

MPH01225 150 ul / 10 uM
EUR 145.2

hsa-miR-195 Primers

MPH01226 150 ul / 10 uM
EUR 145.2

hsa-miR-197 Primers

MPH01229 150 ul / 10 uM
EUR 145.2

hsa-miR-198 Primers

MPH01238 150 ul / 10 uM
EUR 145.2

hsa-miR-19a Primers

MPH01242 150 ul / 10 uM
EUR 145.2

hsa-miR-19b Primers

MPH01243 150 ul / 10 uM
EUR 145.2

hsa-miR-202 Primers

MPH01247 150 ul / 10 uM
EUR 145.2

hsa-miR-203 Primers

MPH01248 150 ul / 10 uM
EUR 145.2

hsa-miR-204 Primers

MPH01249 150 ul / 10 uM
EUR 145.2

hsa-miR-205 Primers

MPH01250 150 ul / 10 uM
EUR 145.2

hsa-miR-206 Primers

MPH01254 150 ul / 10 uM
EUR 145.2

hsa-miR-20a Primers

MPH01257 150 ul / 10 uM
EUR 145.2

hsa-miR-20b Primers

MPH01258 150 ul / 10 uM
EUR 145.2

hsa-miR-210 Primers

MPH01260 150 ul / 10 uM
EUR 145.2

hsa-miR-211 Primers

MPH01261 150 ul / 10 uM
EUR 145.2

hsa-miR-212 Primers

MPH01268 150 ul / 10 uM
EUR 145.2

hsa-miR-214 Primers

MPH01269 150 ul / 10 uM
EUR 145.2

hsa-miR-215 Primers

MPH01270 150 ul / 10 uM
EUR 145.2

hsa-miR-218 Primers

MPH01273 150 ul / 10 uM
EUR 145.2

hsa-miR-221 Primers

MPH01281 150 ul / 10 uM
EUR 145.2

hsa-miR-222 Primers

MPH01282 150 ul / 10 uM
EUR 145.2

hsa-miR-223 Primers

MPH01283 150 ul / 10 uM
EUR 145.2

hsa-miR-224 Primers

MPH01284 150 ul / 10 uM
EUR 145.2

hsa-miR-23a Primers

MPH01291 150 ul / 10 uM
EUR 145.2

hsa-miR-23b Primers

MPH01292 150 ul / 10 uM
EUR 145.2

hsa-miR-23c Primers

MPH01293 150 ul / 10 uM
EUR 145.2

hsa-miR-26a Primers

MPH01296 150 ul / 10 uM
EUR 145.2

hsa-miR-26b Primers

MPH01297 150 ul / 10 uM
EUR 145.2

hsa-miR-27a Primers

MPH01298 150 ul / 10 uM
EUR 145.2

hsa-miR-27b Primers

MPH01299 150 ul / 10 uM
EUR 145.2

hsa-miR-297 Primers

MPH01306 150 ul / 10 uM
EUR 145.2

hsa-miR-298 Primers

MPH01307 150 ul / 10 uM
EUR 145.2

hsa-miR-29a Primers

MPH01310 150 ul / 10 uM
EUR 145.2

hsa-miR-29b Primers

MPH01311 150 ul / 10 uM
EUR 145.2

hsa-miR-29c Primers

MPH01312 150 ul / 10 uM
EUR 145.2

hsa-miR-300 Primers

MPH01313 150 ul / 10 uM
EUR 145.2

hsa-miR-30a Primers

MPH01324 150 ul / 10 uM
EUR 145.2

hsa-miR-30b Primers

MPH01325 150 ul / 10 uM
EUR 145.2

hsa-miR-30c Primers

MPH01326 150 ul / 10 uM
EUR 145.2

hsa-miR-30d Primers

MPH01327 150 ul / 10 uM
EUR 145.2

hsa-miR-30e Primers

MPH01328 150 ul / 10 uM
EUR 145.2

hsa-miR-325 Primers

MPH01431 150 ul / 10 uM
EUR 145.2

hsa-miR-326 Primers

MPH01432 150 ul / 10 uM
EUR 145.2

hsa-miR-328 Primers

MPH01433 150 ul / 10 uM
EUR 145.2

hsa-miR-329 Primers

MPH01434 150 ul / 10 uM
EUR 145.2

hsa-miR-335 Primers

MPH01439 150 ul / 10 uM
EUR 145.2

hsa-miR-33a Primers

MPH01446 150 ul / 10 uM
EUR 145.2

hsa-miR-33b Primers

MPH01447 150 ul / 10 uM
EUR 145.2

hsa-miR-340 Primers

MPH01448 150 ul / 10 uM
EUR 145.2

hsa-miR-345 Primers

MPH01451 150 ul / 10 uM
EUR 145.2

hsa-miR-346 Primers

MPH01452 150 ul / 10 uM
EUR 145.2

hsa-miR-34a Primers

MPH01453 150 ul / 10 uM
EUR 145.2

hsa-miR-34b Primers

MPH01454 150 ul / 10 uM
EUR 145.2

The main aspects of forming the current distribution of taxa involved in the colonization of the island, especially the new habitat and inter-island colonization habitat are largely the same, with Gran Canaria and Tenerife as a major source of diversification and further colonization events. Pedrosia part is the most diverse in terms of the events of colonization, the number of species and habitat heterogeneity, including events in back-colonization of the continent. Subdivision in Pedrosia radiated into diverse habitats recently (late Pleistocene, ca 0.23 to 0.29 Ma) and additional molecular markers and sampling will be necessary to understand the events of the most latest deployment of this group in the Canary Islands and the Cape Verde.

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