Molecular phylogenetic position of Minamitalitrus zoltani elucidates a further troglobisation pattern in cave-dwelling terrestrial amphipods (Crustacea: Talitridae)

Talitrids is a very diverse group of amphipod crustaceans that have been colonized by a variety of terrestrial habitats. Three genera have been successfully adapted to cave habitats on islands in the Pacific and Atlantic Oceans. However, the evolutionary origin of the Pacific talitrids troglobitic remain unknown. We estimate the phylogenetic position of Minamitalitrus zoltani troglobitic, which inhabits limestone cave in Minamidaito Island in the Northwestern Pacific, on the basis of the traditional multi-locus datasets. For talitrids analyzed, we also reconstruct the ancestral homeland of palp maxilliped and gnathopod male 2.

Our results show that Minamitalitrus zoltani is sister to curvatus Nipponorchestia epigean with profound differences. Nipponorchestia curvatus inhabit coastal habitats in Japan, but it is not customary for Minamidaito Island. Previous research estimates that the Atlantic troglobitic species have invaded the underground habitat several times, but we provide new insights into the history troglobisation in talitrids. We also recover secondary shift state palp character maxilliped and men gnathopod 2 in the lineage consists of Minamitalitrus and genera phylogenetically closely.

Our findings highlight the need for reclassification genus-the genus level; Nipponorchestia we split into two genera, establish a new genus for Nipponorchestia nudiramus. A phylogenetic analysis of genera of strongyloid Cloacininae sub-family of marsupials macropodoid in Australasia is based on morphological characteristics and sequence analysis continued (+ ITS) first (ITS-1) and second (ITS-2) internal transcribed spacer of ribosomal DNA. Both approaches are provided robust phylogeny, but the similarities between the two methods in terms of generic grouping suggests that needed substantial revision of the classification phenetic this time, with some morphological characteristics of the current key used for defining genera and tribes prove to be homoplasious.

Molecular phylogenetic, population genetic and demographic studies and Nodularia Nodularia douglasiae breviconcha based CO1 and 16S rRNA

freshwater mussels belonging to the genus Nodularia (Family Unionidae) is known to be widely distributed in East Asia. Although phylogenetic and population genetic studies have been conducted on this species, remains unresolved questions in the taxonomic status and biogeographic distribution channels. Here, the nucleotide sequence of 16S rRNA CO1 and a newly defined than 86 N. douglasiae and 83 individuals N. breviconcha collected on the Korean Peninsula.

Based on these data, we revealed the following results: (1) N. douglasiae can be divided into three genetic clades A (found only on the Korean Peninsula), B (widely distributed in East Asia), and C (found only in western China and Russian), (2) a clade A is not an independent species but concrete member N. douglasiae given the lack of genetic differences between clades A and B, and (3) N. breviconcha not subspecies N. douglasiae but independent species apart from N. douglasiae. In addition, we suggest plausible scenario events biogeographic distribution and demographic history Nodularia species.

 Molecular phylogenetic position of Minamitalitrus zoltani elucidates a further troglobisation pattern in cave-dwelling terrestrial amphipods (Crustacea: Talitridae)
Molecular phylogenetic position of Minamitalitrus zoltani elucidates a further troglobisation pattern in cave-dwelling terrestrial amphipods (Crustacea: Talitridae)

molecular phylogenetics of Lotus (Leguminosae) with an emphasis in tempo and pattern of colonization in the region Macaronesian

With a wide variety of distribution including Europe and Asia, Lotus (Leguminosae) is the largest genus in Loteae. This is especially diverse in the Mediterreanean and the five islands of Macaronesia (the Atlantic Ocean). However, little is known about the relationship between section 14 currently recognized in the Lotus and about the timing and pattern of colonization in the Macaronesian region. In this study, we used the four regions of DNA (ribosomal nuclear ITS plus three regions plastid) in sampling comprehensively most species Lotus to date (some endemic species in the Canary Islands which are under-represented in the phylogenetic analysis before) to infer relationships within this genus and for establishing patterns of colonization in Macaronesia.

Divergence time estimates and habitat reconstruction analysis showed that Lotus possibility diverged about 7.86 Ma of the sister group, but all the events of colonization to occur Macaronesia recently (starting from the latter 0.23 to 2.70 Ma). Diversification Lotus in Macaronesia involved between four and six independent colonization events of the four sections is currently distributed in Africa and Europe.

mmu-let-7c Primers

MPM00009 150 ul / 10 uM
EUR 121

Mouse pre-microRNA Expression Construct let-7c-1

MMIR-let-7c-1-PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

Mouse pre-microRNA Expression Construct let-7c-2

MMIR-let-7c-2-PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

rno-let-7c-5p Primers

MPR00312 150 ul / 10 uM
EUR 176

mmu-let-7c-5p Primers

MPM00741 150 ul / 10 uM
EUR 121

hsa-let-7c miRNA Inhibitor

MIH01007 2 x 5.0 nmol
EUR 176

hsa-let-7c miRNA Antagomir

MNH01007 2 x 5.0 nmol
EUR 329

rno-let-7c-1-3p Primers

MPR00310 150 ul / 10 uM
EUR 176

rno-let-7c-2-3p Primers

MPR00311 150 ul / 10 uM
EUR 176

mmu-let-7c-1-3p Primers

MPM00739 150 ul / 10 uM
EUR 121

mmu-let-7c-2-3p Primers

MPM00740 150 ul / 10 uM
EUR 121

hsa-let-7c-3p miRNA Inhibitor

MIH01008 2 x 5.0 nmol
EUR 176

hsa-let-7c-5p miRNA Inhibitor

MIH01009 2 x 5.0 nmol
EUR 176

hsa-let-7c-3p miRNA Antagomir

MNH01008 2 x 5.0 nmol
EUR 329

hsa-let-7c-5p miRNA Antagomir

MNH01009 2 x 5.0 nmol
EUR 329

hsa-let-7a Primers

MPH01006 150 ul / 10 uM
EUR 121

hsa-let-7b Primers

MPH01007 150 ul / 10 uM
EUR 121

hsa-let-7d Primers

MPH01009 150 ul / 10 uM
EUR 121

hsa-let-7e Primers

MPH01010 150 ul / 10 uM
EUR 121

hsa-let-7f Primers

MPH01011 150 ul / 10 uM
EUR 121

hsa-let-7g Primers

MPH01012 150 ul / 10 uM
EUR 121

hsa-let-7i Primers

MPH01013 150 ul / 10 uM
EUR 121

hsa-let-7c RT-PCR Detection Kit

20-abx096017
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  • EUR 732.00
  • EUR 398.00
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hsa-let-7c RT-PCR Primer Set

abx096771-1nmol 1 nmol
EUR 286
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hsa-let-7a-3p Primers

MPH02041 150 ul / 10 uM
EUR 121

hsa-let-7a-5p Primers

MPH02042 150 ul / 10 uM
EUR 121

hsa-let-7b-3p Primers

MPH02043 150 ul / 10 uM
EUR 121

hsa-let-7b-5p Primers

MPH02044 150 ul / 10 uM
EUR 121

hsa-let-7d-3p Primers

MPH02047 150 ul / 10 uM
EUR 121

hsa-let-7d-5p Primers

MPH02048 150 ul / 10 uM
EUR 121

hsa-let-7e-3p Primers

MPH02049 150 ul / 10 uM
EUR 121

hsa-let-7e-5p Primers

MPH02050 150 ul / 10 uM
EUR 121

hsa-let-7f-5p Primers

MPH02053 150 ul / 10 uM
EUR 121

hsa-let-7g-3p Primers

MPH02054 150 ul / 10 uM
EUR 121

hsa-let-7g-5p Primers

MPH02055 150 ul / 10 uM
EUR 121

hsa-let-7i-3p Primers

MPH02056 150 ul / 10 uM
EUR 121

hsa-let-7i-5p Primers

MPH02057 150 ul / 10 uM
EUR 121

hsa-let-7f-1-3p Primers

MPH02051 150 ul / 10 uM
EUR 121

hsa-let-7f-2-3p Primers

MPH02052 150 ul / 10 uM
EUR 121

hsa-let-7a-2-3p Primers

MPH03980 150 ul / 10 uM
EUR 121

mmu-let-7c miRNA Antagomir

MNM01007 2 x 5.0 nmol
EUR 329

rno-let-7c miRNA Antagomir

MNR01007 2 x 5.0 nmol
EUR 329

mmu-let-7c miRNA Inhibitor

MIM01007 2 x 5.0 nmol
EUR 176

rno-let-7c miRNA Inhibitor

MIR01007 2 x 5.0 nmol
EUR 176

hsa-let-7c RT-PCR Detection and U6 Calibration Kit

20-abx096394
  • EUR 690.00
  • EUR 954.00
  • EUR 495.00
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  • Shipped within 5-10 working days.

mmu-let-7c-5p miRNA Antagomir

MNM01010 2 x 5.0 nmol
EUR 329

rno-let-7c-5p miRNA Antagomir

MNR01010 2 x 5.0 nmol
EUR 329

mmu-let-7c-5p miRNA Inhibitor

MIM01010 2 x 5.0 nmol
EUR 176

rno-let-7c-5p miRNA Inhibitor

MIR01010 2 x 5.0 nmol
EUR 176

mmu-let-7c-1-3p miRNA Antagomir

MNM01008 2 x 5.0 nmol
EUR 329

mmu-let-7c-2-3p miRNA Antagomir

MNM01009 2 x 5.0 nmol
EUR 329

rno-let-7c-1-3p miRNA Antagomir

MNR01008 2 x 5.0 nmol
EUR 329

rno-let-7c-2-3p miRNA Antagomir

MNR01009 2 x 5.0 nmol
EUR 329

mmu-let-7c-1-3p miRNA Inhibitor

MIM01008 2 x 5.0 nmol
EUR 176

mmu-let-7c-2-3p miRNA Inhibitor

MIM01009 2 x 5.0 nmol
EUR 176

rno-let-7c-1-3p miRNA Inhibitor

MIR01008 2 x 5.0 nmol
EUR 176

rno-let-7c-2-3p miRNA Inhibitor

MIR01009 2 x 5.0 nmol
EUR 176

Human pre-microRNA Expression Construct let-7c

PMIRHlet7cPA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

rno-let-7d Primers

MP-r00007 150 ul / 10 uM
EUR 176

rno-let-7a Primers

MP-r00033 150 ul / 10 uM
EUR 176

rno-let-7b Primers

MP-r00034 150 ul / 10 uM
EUR 176

rno-let-7e Primers

MP-r00036 150 ul / 10 uM
EUR 176

rno-let-7f Primers

MP-r00037 150 ul / 10 uM
EUR 176

rno-let-7i Primers

MP-r00038 150 ul / 10 uM
EUR 176

mmu-let-7a Primers

MPM00007 150 ul / 10 uM
EUR 121

mmu-let-7b Primers

MPM00008 150 ul / 10 uM
EUR 121

mmu-let-7d Primers

MPM00010 150 ul / 10 uM
EUR 121

mmu-let-7e Primers

MPM00011 150 ul / 10 uM
EUR 121

mmu-let-7f Primers

MPM00012 150 ul / 10 uM
EUR 121

mmu-let-7g Primers

MPM00013 150 ul / 10 uM
EUR 121

mmu-let-7i Primers

MPM00014 150 ul / 10 uM
EUR 121

mmu-let-7j Primers

MPM00753 150 ul / 10 uM
EUR 121

mmu-let-7k Primers

MPM00754 150 ul / 10 uM
EUR 121

rno-let-7a-5p Primers

MPR00307 150 ul / 10 uM
EUR 176

rno-let-7b-3p Primers

MPR00308 150 ul / 10 uM
EUR 176

rno-let-7b-5p Primers

MPR00309 150 ul / 10 uM
EUR 176

rno-let-7d-3p Primers

MPR00313 150 ul / 10 uM
EUR 176

rno-let-7d-5p Primers

MPR00314 150 ul / 10 uM
EUR 176

rno-let-7e-3p Primers

MPR00315 150 ul / 10 uM
EUR 176

rno-let-7e-5p Primers

MPR00316 150 ul / 10 uM
EUR 176

rno-let-7f-5p Primers

MPR00319 150 ul / 10 uM
EUR 176

rno-let-7g-3p Primers

MPR00320 150 ul / 10 uM
EUR 176

rno-let-7g-5p Primers

MPR00321 150 ul / 10 uM
EUR 176

rno-let-7i-3p Primers

MPR00322 150 ul / 10 uM
EUR 176

rno-let-7i-5p Primers

MPR00323 150 ul / 10 uM
EUR 176

mmu-let-7a-5p Primers

MPM00736 150 ul / 10 uM
EUR 121

mmu-let-7b-3p Primers

MPM00737 150 ul / 10 uM
EUR 121

mmu-let-7b-5p Primers

MPM00738 150 ul / 10 uM
EUR 121

mmu-let-7d-3p Primers

MPM00742 150 ul / 10 uM
EUR 121

mmu-let-7d-5p Primers

MPM00743 150 ul / 10 uM
EUR 121

mmu-let-7e-3p Primers

MPM00744 150 ul / 10 uM
EUR 121

mmu-let-7e-5p Primers

MPM00745 150 ul / 10 uM
EUR 121

mmu-let-7f-5p Primers

MPM00748 150 ul / 10 uM
EUR 121

mmu-let-7g-3p Primers

MPM00749 150 ul / 10 uM
EUR 121

mmu-let-7g-5p Primers

MPM00750 150 ul / 10 uM
EUR 121

mmu-let-7i-3p Primers

MPM00751 150 ul / 10 uM
EUR 121

mmu-let-7i-5p Primers

MPM00752 150 ul / 10 uM
EUR 121

hsa-let-7c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097605
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

Dinitrophenyl (DNP)-HSA protein Conjugate

AV-9330-HSA 10 mg
EUR 286

hsa-let-7a miRNA Inhibitor

MIH01000 2 x 5.0 nmol
EUR 176

hsa-let-7b miRNA Inhibitor

MIH01004 2 x 5.0 nmol
EUR 176

hsa-let-7d miRNA Inhibitor

MIH01010 2 x 5.0 nmol
EUR 176

hsa-let-7e miRNA Inhibitor

MIH01013 2 x 5.0 nmol
EUR 176

hsa-let-7g miRNA Inhibitor

MIH01020 2 x 5.0 nmol
EUR 176

hsa-let-7i miRNA Inhibitor

MIH01023 2 x 5.0 nmol
EUR 176

hsa-let-7a miRNA Antagomir

MNH01000 2 x 5.0 nmol
EUR 329

hsa-let-7b miRNA Antagomir

MNH01004 2 x 5.0 nmol
EUR 329

hsa-let-7d miRNA Antagomir

MNH01010 2 x 5.0 nmol
EUR 329

hsa-let-7e miRNA Antagomir

MNH01013 2 x 5.0 nmol
EUR 329

hsa-let-7f miRNA Antagomir

MNH01016 2 x 5.0 nmol
EUR 329

hsa-let-7g miRNA Antagomir

MNH01020 2 x 5.0 nmol
EUR 329

hsa-let-7i miRNA Antagomir

MNH01023 2 x 5.0 nmol
EUR 329

pLL3.7- Hsa- Let- 7a- 1miRNA

PVT10410 2 ug
EUR 301

rno-let-7a-2-3p Primers

MPR00306 150 ul / 10 uM
EUR 176

rno-let-7f-1-3p Primers

MPR00317 150 ul / 10 uM
EUR 176

rno-let-7f-2-3p Primers

MPR00318 150 ul / 10 uM
EUR 176

rno-let-7a-1-3p Primers

MPR00878 150 ul / 10 uM
EUR 176

mmu-let-7a-1-3p Primers

MPM00734 150 ul / 10 uM
EUR 121

mmu-let-7a-2-3p Primers

MPM00735 150 ul / 10 uM
EUR 121

mmu-let-7f-1-3p Primers

MPM00746 150 ul / 10 uM
EUR 121

mmu-let-7f-2-3p Primers

MPM00747 150 ul / 10 uM
EUR 121

hsa-let-7a-3p miRNA Inhibitor

MIH01002 2 x 5.0 nmol
EUR 176

hsa-let-7a-5p miRNA Inhibitor

MIH01003 2 x 5.0 nmol
EUR 176

hsa-let-7b-3p miRNA Inhibitor

MIH01005 2 x 5.0 nmol
EUR 176

hsa-let-7b-5p miRNA Inhibitor

MIH01006 2 x 5.0 nmol
EUR 176

hsa-let-7d-3p miRNA Inhibitor

MIH01011 2 x 5.0 nmol
EUR 176

hsa-let-7d-5p miRNA Inhibitor

MIH01012 2 x 5.0 nmol
EUR 176

hsa-let-7e-3p miRNA Inhibitor

MIH01014 2 x 5.0 nmol
EUR 176

hsa-let-7e-5p miRNA Inhibitor

MIH01015 2 x 5.0 nmol
EUR 176

hsa-let-7f-5p miRNA Inhibitor

MIH01019 2 x 5.0 nmol
EUR 176

hsa-let-7g-3p miRNA Inhibitor

MIH01021 2 x 5.0 nmol
EUR 176

hsa-let-7g-5p miRNA Inhibitor

MIH01022 2 x 5.0 nmol
EUR 176

hsa-let-7i-3p miRNA Inhibitor

MIH01024 2 x 5.0 nmol
EUR 176

hsa-let-7i-5p miRNA Inhibitor

MIH01025 2 x 5.0 nmol
EUR 176

hsa-let-7a-3p miRNA Antagomir

MNH01002 2 x 5.0 nmol
EUR 329

hsa-let-7a-5p miRNA Antagomir

MNH01003 2 x 5.0 nmol
EUR 329

hsa-let-7b-3p miRNA Antagomir

MNH01005 2 x 5.0 nmol
EUR 329

hsa-let-7b-5p miRNA Antagomir

MNH01006 2 x 5.0 nmol
EUR 329

hsa-let-7d-3p miRNA Antagomir

MNH01011 2 x 5.0 nmol
EUR 329

hsa-let-7d-5p miRNA Antagomir

MNH01012 2 x 5.0 nmol
EUR 329

hsa-let-7e-3p miRNA Antagomir

MNH01014 2 x 5.0 nmol
EUR 329

hsa-let-7e-5p miRNA Antagomir

MNH01015 2 x 5.0 nmol
EUR 329

hsa-let-7f-5p miRNA Antagomir

MNH01019 2 x 5.0 nmol
EUR 329

hsa-let-7g-3p miRNA Antagomir

MNH01021 2 x 5.0 nmol
EUR 329

hsa-let-7g-5p miRNA Antagomir

MNH01022 2 x 5.0 nmol
EUR 329

hsa-let-7i-3p miRNA Antagomir

MNH01024 2 x 5.0 nmol
EUR 329

hsa-let-7i-5p miRNA Antagomir

MNH01025 2 x 5.0 nmol
EUR 329

Mouse pre-microRNA Expression Construct let-7b

MMIR-let-7b-PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

Mouse pre-microRNA Expression Construct let-7d

MMIR-let-7d-PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

Mouse pre-microRNA Expression Construct let-7e

MMIR-let-7e-PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

Mouse pre-microRNA Expression Construct let-7g

MMIR-let-7g-PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

hsa-let-7a RT-PCR Detection Kit

20-abx096015
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

hsa-let-7b RT-PCR Detection Kit

20-abx096016
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

hsa-let-7d RT-PCR Detection Kit

20-abx096018
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
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  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

hsa-let-7e RT-PCR Detection Kit

20-abx096019
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

hsa-let-7f RT-PCR Detection Kit

20-abx096020
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

hsa-let-7g RT-PCR Detection Kit

20-abx096021
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

hsa-let-7i RT-PCR Detection Kit

20-abx096022
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

hsa-let-7f RT-PCR Detection Kit

20-abx096023
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

hsa-let-7a RT-PCR Primer Set

abx096769-1nmol 1 nmol
EUR 286
  • Shipped within 5-10 working days.

hsa-let-7b RT-PCR Primer Set

abx096770-1nmol 1 nmol
EUR 286
  • Shipped within 5-10 working days.

hsa-let-7d RT-PCR Primer Set

abx096772-1nmol 1 nmol
EUR 286
  • Shipped within 5-10 working days.

hsa-let-7e RT-PCR Primer Set

abx096773-1nmol 1 nmol
EUR 286
  • Shipped within 5-10 working days.

hsa-let-7f RT-PCR Primer Set

abx096774-1nmol 1 nmol
EUR 286
  • Shipped within 5-10 working days.

hsa-let-7g RT-PCR Primer Set

abx096775-1nmol 1 nmol
EUR 286
  • Shipped within 5-10 working days.

hsa-let-7i RT-PCR Primer Set

abx096776-1nmol 1 nmol
EUR 286
  • Shipped within 5-10 working days.

hsa-let-7f RT-PCR Primer Set

abx096777-1nmol 1 nmol
EUR 286
  • Shipped within 5-10 working days.

hsa-let-7a-2-3p miRNA Inhibitor

MIH01001 2 x 5.0 nmol
EUR 176

hsa-let-7f-1-3p miRNA Inhibitor

MIH01017 2 x 5.0 nmol
EUR 176

hsa-let-7f-2-3p miRNA Inhibitor

MIH01018 2 x 5.0 nmol
EUR 176

hsa-let-7a-2-3p miRNA Antagomir

MNH01001 2 x 5.0 nmol
EUR 329

hsa-let-7f-1-3p miRNA Antagomir

MNH01017 2 x 5.0 nmol
EUR 329

hsa-let-7f-2-3p miRNA Antagomir

MNH01018 2 x 5.0 nmol
EUR 329

115-7C

20-abx076785
  • EUR 829.00
  • EUR 342.00
  • 25 mg
  • 5 mg
  • Shipped within 5-12 working days.

The main aspects of forming the current distribution of taxa involved in the colonization of the island, especially the new habitat and inter-island colonization habitat are largely the same, with Gran Canaria and Tenerife as a major source of diversification and further colonization events. Pedrosia part is the most diverse in terms of the events of colonization, the number of species and habitat heterogeneity, including events in back-colonization of the continent. Subdivision in Pedrosia radiated into diverse habitats recently (late Pleistocene, ca 0.23 to 0.29 Ma) and additional molecular markers and sampling will be necessary to understand the events of the most latest deployment of this group in the Canary Islands and the Cape Verde.

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