Ctenophthalmus baeticus boisseauorum (Beaucournu, 1968) and Ctenophthalmus apertus allani (Smit, 1955) (Siphonaptera: Ctenophthalmidae) as synonymous taxa: morphometric, phylogenetic, and molecular characterization.

Family Ctenophthalmidae (Order Siphonaptera) has been regarded as a ‘catchall’ for a variety of divergent taxa shows paraphyletic origin. In turn, Ctenophthalmus sp. (Ctenophthalmidae) including 300 valid taxa described. In this genus, men easily distinguished based on the size, shape, and chaetotaxy their genitals; However, women showed little morphological differences with each other. The main objective of this research is to carry out morphometric comparative, phylogenetic and molecular studies of two distinct subspecies: Ctenophthalmus baeticus boisseauorum and Ctenophthalmus apertus allani to clarify and discuss its taxonomic status. From the point of morphological and biometrical point of view, we find a clear distinction between abdominal segments modification of the men of the two subspecies and a slight difference in the margin of the sternum VII of all specimens of women who are not in accordance with the results of molecular and phylogenetic based on four molecular markers of different ( mediakan internal Spacer 1 and 2 of ribosomal DNA, and partial cytochrome c oxidase subunit 1 mitochondrial DNA and cytochrome b).

Thus, we observed phenotypic plasticity between the two subspecies, which does not correspond to the real genotype variability or environmental or ecological conditions are different. Basing on these results, we can assume that there is no strong argument to consider two ‘morphosubspecies’ as two distinct taxa. We propose that C. b. boisseauorum should be considered a junior synonym of C. a. allani.Accumulations investigated potentially toxic metals in the soil, and five species of North Caucasian regions Alyssum of metalliferous and non-metalliferous areas in Karachay-Cherkessia, Kabardino-Balkaria, Dagestan and the Krasnodar region.

The analysis of field samples showed that the chemical features of the soil significantly affect concentrations of Ni, Co, Zn, but it has less effect on the concentration of Cu and Pb in the shoots of Alyssum. Variations in the rate of gathering capabilities found in the species studied, including hiperakumulasi Ni in Alyssum murale (up to 12,100 mg kg-1), and a significant accumulation of Zn in A. gehamense (up to 1700 mg kg-1). A comparative molecular genetic analysis of two populations of A. murale, both residents of Ni-hyperaccumulating of Karachay-Cherkessia and non-hyperaccumulating population of Dagestan, showed a fairly large genetic differences between them. These results support the hypothesis that the selection of the metal hiperakumulator species with enhanced phytoremediation efficiency should be considered at the population level.

A survey of molecular and phylogenetic analysis of pig Circovirus type 3 using oral fluid, faeces and serum

Background: Porcine Circovirus Circovirus type 3 is the most recently discovered hog and emerging pathogens. In this study the presence status on some farms Slovenia reported. The effectiveness of the vaccine against swine Circovirus type 2 assessed against swine Circovirus type of sample Group 3. Oral fluid, faeces and individual serum samples taken from six different categories of pigs and tested for the presence of viral DNA, using both real-time and conventional PCR. positive samples were subjected to direct Sanger sequencing. The nucleotide sequences were analyzed and compared with GenBank sequences PCV3.

Results: Positive samples were sent for genome sequencing, which confirmed the presence of the virus in all categories of pigs on five different farms. A high to moderate correlation strong statistical significance was found between individual serum samples, oral fluid and feces. Slovenia PCV3 found to be distributed in the same way with other countries. Slovenia PCV3 nt sequence highly related, share more than 99.5% nt identity.

Molecular characterization and phylogenetic analysis of Anopheles (Anophelinae: Culicidae) mosquitoes of Oriental and Afrotropical zone Zoogeographic in Saudi Arabia.

The Kingdom of Saudi Arabia (KSA) has a diverse fauna due to the strange position bordering the Afrotropical, Oriental and Palaearctic zoogeographic zone. This study reports on the phylogenetic of five species belonging to five series of Anopheles mosquitoes (Cellia). We collect mosquito larvae from the east, west and southwest of KSA. mosquito samples were identified using morphological image button mosquitoes and characterized by using a single analysis and multi-locus-internal transcribed spacer 2 (ITS2) region and cytochrome c oxidase subunit I (COI).

Based on morphological data and molecular five recognized species, such as An. stephensi (Neocellia) (Oriental), An. arabiensis (Pyretophorus) (Afrotropical), An. dthali (Myzomyia) (Oriental and Palaearctic), An. cinereus (Paramyzomyia) and An. rhodesiensis Rupicola (Neomyzomyia) (Oriental and Palaearctic). Phylogenetic analysis showed that An. stephensi is monophyletic species with different ecotypes are found in different geographic regions. Comprehensive phylogenetic and population genetics studies are very important for a better understanding of the role of these five species of mosquitoes in malaria transmission in various zoogeographic zones ecological characteristics and a different demographic.

hsa-let-7f-1-3p Primers
MPH02051	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7f-2-3p Primers
MPH02052	ABM	150 ul / 10 uM	EUR 145.2

rno-let-7f Primers
MP-r00037	ABM	150 ul / 10 uM	EUR 211.2

mmu-let-7f Primers
MPM00012	ABM	150 ul / 10 uM	EUR 145.2

rno-let-7f-5p Primers
MPR00319	ABM	150 ul / 10 uM	EUR 211.2

mmu-let-7f-5p Primers
MPM00748	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7a Primers
MPH01006	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7b Primers
MPH01007	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7c Primers
MPH01008	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7d Primers
MPH01009	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7e Primers
MPH01010	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7g Primers
MPH01012	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7i Primers
MPH01013	ABM	150 ul / 10 uM	EUR 145.2

rno-let-7f-1-3p Primers
MPR00317	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7f-2-3p Primers
MPR00318	ABM	150 ul / 10 uM	EUR 211.2

mmu-let-7f-1-3p Primers
MPM00746	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7f-2-3p Primers
MPM00747	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7a-3p Primers
MPH02041	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7a-5p Primers
MPH02042	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7b-3p Primers
MPH02043	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7b-5p Primers
MPH02044	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7c-3p Primers
MPH02045	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7c-5p Primers
MPH02046	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7d-3p Primers
MPH02047	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7d-5p Primers
MPH02048	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7e-3p Primers
MPH02049	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7e-5p Primers
MPH02050	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7g-3p Primers
MPH02054	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7g-5p Primers
MPH02055	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7i-3p Primers
MPH02056	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7i-5p Primers
MPH02057	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7a-2-3p Primers
MPH03980	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7f RT-PCR Primer Set
abx096774-1nmol	Abbexa	1 nmol	EUR 343.2

hsa-let-7f RT-PCR Primer Set
abx096777-1nmol	Abbexa	1 nmol	EUR 343.2

rno-let-7d Primers
MP-r00007	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7a Primers
MP-r00033	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7b Primers
MP-r00034	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7c Primers
MP-r00035	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7e Primers
MP-r00036	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7i Primers
MP-r00038	ABM	150 ul / 10 uM	EUR 211.2

mmu-let-7a Primers
MPM00007	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7b Primers
MPM00008	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7c Primers
MPM00009	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7d Primers
MPM00010	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7e Primers
MPM00011	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7g Primers
MPM00013	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7i Primers
MPM00014	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7j Primers
MPM00753	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7k Primers
MPM00754	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-1 Primers
MPH01014	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-7 Primers
MPH01976	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-9 Primers
MPH02015	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-16 Primers
MPH01191	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-17 Primers
MPH01192	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-21 Primers
MPH01259	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-22 Primers
MPH01277	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-24 Primers
MPH01294	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-25 Primers
MPH01295	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-31 Primers
MPH01329	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-32 Primers
MPH01417	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-93 Primers
MPH02022	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-95 Primers
MPH02035	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-96 Primers
MPH02036	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-98 Primers
MPH02037	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-100 Primers
MPH01015	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-101 Primers
MPH01016	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-103 Primers
MPH01017	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-105 Primers
MPH01019	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-107 Primers
MPH01022	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-10a Primers
MPH01023	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-10b Primers
MPH01024	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-122 Primers
MPH01045	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-124 Primers
MPH01060	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-126 Primers
MPH01082	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-128 Primers
MPH01110	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-132 Primers
MPH01144	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-134 Primers
MPH01151	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-136 Primers
MPH01154	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-137 Primers
MPH01155	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-138 Primers
MPH01156	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-141 Primers
MPH01161	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-143 Primers
MPH01164	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-144 Primers
MPH01165	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-145 Primers
MPH01166	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-147 Primers
MPH01172	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-149 Primers
MPH01178	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-150 Primers
MPH01179	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-152 Primers
MPH01182	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-153 Primers
MPH01183	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-154 Primers
MPH01187	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-155 Primers
MPH01188	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-15a Primers
MPH01189	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-15b Primers
MPH01190	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-182 Primers
MPH01197	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-183 Primers
MPH01201	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-184 Primers
MPH01202	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-185 Primers
MPH01203	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-186 Primers
MPH01204	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-187 Primers
MPH01205	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-18a Primers
MPH01208	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-18b Primers
MPH01209	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-190 Primers
MPH01210	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-191 Primers
MPH01214	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-192 Primers
MPH01221	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-194 Primers
MPH01225	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-195 Primers
MPH01226	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-197 Primers
MPH01229	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-198 Primers
MPH01238	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-19a Primers
MPH01242	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-19b Primers
MPH01243	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-202 Primers
MPH01247	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-203 Primers
MPH01248	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-204 Primers
MPH01249	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-205 Primers
MPH01250	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-206 Primers
MPH01254	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-20a Primers
MPH01257	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-20b Primers
MPH01258	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-210 Primers
MPH01260	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-211 Primers
MPH01261	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-212 Primers
MPH01268	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-214 Primers
MPH01269	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-215 Primers
MPH01270	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-218 Primers
MPH01273	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-221 Primers
MPH01281	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-222 Primers
MPH01282	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-223 Primers
MPH01283	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-224 Primers
MPH01284	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-23a Primers
MPH01291	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-23b Primers
MPH01292	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-23c Primers
MPH01293	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-26a Primers
MPH01296	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-26b Primers
MPH01297	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-27a Primers
MPH01298	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-27b Primers
MPH01299	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-297 Primers
MPH01306	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-298 Primers
MPH01307	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-29a Primers
MPH01310	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-29b Primers
MPH01311	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-29c Primers
MPH01312	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-300 Primers
MPH01313	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-30a Primers
MPH01324	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-30b Primers
MPH01325	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-30c Primers
MPH01326	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-30d Primers
MPH01327	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-30e Primers
MPH01328	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-325 Primers
MPH01431	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-326 Primers
MPH01432	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-328 Primers
MPH01433	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-329 Primers
MPH01434	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-335 Primers
MPH01439	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-33a Primers
MPH01446	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-33b Primers
MPH01447	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-340 Primers
MPH01448	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-345 Primers
MPH01451	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-346 Primers
MPH01452	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-34a Primers
MPH01453	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-34b Primers
MPH01454	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-363 Primers
MPH01486	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-365 Primers
MPH01492	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-367 Primers
MPH01514	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-370 Primers
MPH01545	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-372 Primers
MPH01550	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-373 Primers
MPH01551	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-375 Primers
MPH01555	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-377 Primers
MPH01559	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-378 Primers
MPH01560	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-379 Primers
MPH01563	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-380 Primers
MPH01564	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-381 Primers
MPH01565	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-382 Primers
MPH01566	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-383 Primers
MPH01567	ABM	150 ul / 10 uM	EUR 145.2

There is no current data on genotype Marek’s disease virus (MDV) in poultry Turkey, therefore, this study was conducted to analyze CVI988 / Rispens, turkey herpes virus (HVT) vaccine virus and virus field MDV and to perform phylogenetic analysis of MDV in Turkey chickens.2 layer. In 2017 and 2018, a total of 602 spleen samples collected from 49 layers herd of Marmara, West Black Sea and Aegean regions. DNA was extracted from samples of spleen and samples were analyzed by real-time PCR probes for detecting CVI988 / Rispens and HVT vaccine virus and field MDV strains.