A Report on Molecular Detection and Phylogenetic Evaluation of Anaplasma marginale in Ticks and Blood Samples Collected from Cattle in District Layyah in Punjab (Pakistan)

Anaplasmosis is a tick-borne disease caused by an obligate gram-negative bacteria, Anaplasma (A.) marginale. This study reports on the seasonal prevalence, epidemiology and phylogeny of A. marginale in three breeds of cattle from Layyah district, Southern Punjab, Pakistan. A total of 844 blood samples (Cross = 300, Holstein Friesian = 244, Sahiwal breed = 300) of apparently healthy cattle on a seasonal basis along with the epidemiological data collected during May 2018 to April 2019. The polymerase chain reaction resulting 265 base pair amplicons specific to a major surface 1b protein-encoding genes of A. marginale in 8.6% (73/844) of cattle registered.

The highest prevalence was observed during the fall (18.3%) followed by summer (9.7%) and winter (7.1%). Holstein Friesian breed is most susceptible to infection A. marginale (13.1%) followed by Sahiwal (7.6%) and cross-breeding (6%). partial gene sequences amplified representation of A. marginale submitted to GenBank (Accession number MK032842 and MK032843). 37/844 (4.3%) Giemsa stained blood smears found positive for Anaplasma spp. a small number of ticks including Hyalomma anatolicum, Hyalomma excavatum, Cattle Tick, Haemaphysalis punctata identified from cattle but none of them were found positive PCR for the presence of A. marginale. Epidemiological factor analysis revealed that women with cows and farm water supplies from the pool, other dairy farms where animals and dogs live with cattle and dogs have fleas burden on them had a significant association with the prevalence of A. marginale.

It was observed that the white blood cells, lymphocytes (%), monocytes (%) hematocrit, mean corpuscular hemoglobin and hemoglobin concentration of living cells on average were significantly impaired in A. marginale-positive than negative cows.

Taxonomy and Molecular phylogenetics of Ensiculiferaceae, fam. November (Peridiniales, Dinophyceae), with consideration of their life history

In the current limitation, Thoracosphaeraceae comprises all dinophytes showed calcification coccoid cells produced during the life of their history. Species hitherto assigned to Ensiculifera and Pentapharsodinium largely based on monadoid life-history stages, while links to coccoid stage (sometimes treated taxonomically different) are not always resolved. We investigate the different stages of life-history and the DNA sequence of the data Ensiculifera mexicana and other species occur in samples collected from around the world.

Based on concatenated ribosomal RNA gene sequences represented Ensiculiferaceae peridinalean different branches, which shows the relationship much to dinophytes other calcareous. Both morphological and molecular data (particularly from coccoid stage) revealed the presence of three distinct clades within Ensiculiferaceae, which may include other dinophytes showing the life-history stages of the parasite. At higher taxonomic levels, Ensiculiferaceae shows the relationship of parasites and endosymbionts (eg, Blastodinium and zooxanthella) and dinophytes save diatom not chloroplasts. These unexpected phylogenetic relationships strengthened by the presence of five plates in all taxa Cingular, which is different from the six plates cingular from most other Thoracosphaeraceae. We here describe Ensiculiferaceae, correct description and Pentapharsodinium Ensiculifera, upright Matsuokaea and give some new combinations at the species level.

Molecule Detection of Potato Virus in Bangladesh and their phylogenetic analysis

Potato (Solanum tuberosum) is a major food source all over the world including Bangladesh. viral diseases is a major obstacle to sustainable potato production by reducing the quality and quantity. To determine the current status of the eight important potato virus in Bangladesh, tuber samples were collected from three main potato-growing region (Munshiganj, Jessore and Bogra district) in January-February 2017 and February 2018.

The reverse transcription polymerase chain reaction (RT-PCR) and protein coat (CP) -specific primer used to amplify the CP sequence of each virus, and confirmed by sequencing, which is stored in GenBank. The results showed that the samples of tubers were subjected to virus Potato leafroll (PLRV), potato virus X (PVX), potato virus Y (PVY), virus Potato S (PVS), virus Potato H (PVH), Potato Aucuba mosaic virus (PAMV) and potato virus M (PVM) infection, whereas the mixed infections are very common. Phylogenetic analysis revealed that PLRV from this study is closely related to Canadian and Chinese isolates, respectively; PVX is closely associated with the Canadian and Chinese isolates, respectively; PVY isolates are closely related to China;

mmu-let-7b-3p Primers
MPM00737	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7b-5p Primers
MPM00738	ABM	150 ul / 10 uM	EUR 145.2

rno-let-7b-3p Primers
MPR00308	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7b-5p Primers
MPR00309	ABM	150 ul / 10 uM	EUR 211.2

hsa-let-7b miRNA Antagomir
MNH01004	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7b miRNA Inhibitor
MIH01004	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7a Primers
MPH01006	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7c Primers
MPH01008	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7d Primers
MPH01009	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7e Primers
MPH01010	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7f Primers
MPH01011	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7g Primers
MPH01012	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7i Primers
MPH01013	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7b-3p miRNA Antagomir
MNH01005	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7b-5p miRNA Antagomir
MNH01006	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7b-3p miRNA Inhibitor
MIH01005	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7b-5p miRNA Inhibitor
MIH01006	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7a-3p Primers
MPH02041	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7a-5p Primers
MPH02042	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7c-3p Primers
MPH02045	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7c-5p Primers
MPH02046	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7d-3p Primers
MPH02047	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7d-5p Primers
MPH02048	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7e-3p Primers
MPH02049	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7e-5p Primers
MPH02050	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7f-5p Primers
MPH02053	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7g-3p Primers
MPH02054	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7g-5p Primers
MPH02055	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7i-3p Primers
MPH02056	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7i-5p Primers
MPH02057	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7b RT-PCR Detection Kit
20-abx096016	Abbexa	EUR 644.40 EUR 878.40 EUR 477.60	100 rxns 200 rxns 50 rxns

hsa-let-7b RT-PCR Primer Set
abx096770-1nmol	Abbexa	1 nmol	EUR 343.2

hsa-let-7a-2-3p Primers
MPH03980	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7f-1-3p Primers
MPH02051	ABM	150 ul / 10 uM	EUR 145.2

hsa-let-7f-2-3p Primers
MPH02052	ABM	150 ul / 10 uM	EUR 145.2

rno-let-7b miRNA Antagomir
MNR01004	ABM	2 x 5.0 nmol	EUR 394.8

rno-let-7b miRNA Inhibitor
MIR01004	ABM	2 x 5.0 nmol	EUR 211.2

mmu-let-7b miRNA Antagomir
MNM01004	ABM	2 x 5.0 nmol	EUR 394.8

mmu-let-7b miRNA Inhibitor
MIM01004	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7b RT-PCR Detection and U6 Calibration Kit
20-abx096393	Abbexa	EUR 828.00 EUR 1144.80 EUR 594.00	100 rxns 200 rxns 50 rxns

rno-let-7b-3p miRNA Antagomir
MNR01005	ABM	2 x 5.0 nmol	EUR 394.8

rno-let-7b-5p miRNA Antagomir
MNR01006	ABM	2 x 5.0 nmol	EUR 394.8

rno-let-7b-3p miRNA Inhibitor
MIR01005	ABM	2 x 5.0 nmol	EUR 211.2

rno-let-7b-5p miRNA Inhibitor
MIR01006	ABM	2 x 5.0 nmol	EUR 211.2

mmu-let-7b-3p miRNA Antagomir
MNM01005	ABM	2 x 5.0 nmol	EUR 394.8

mmu-let-7b-5p miRNA Antagomir
MNM01006	ABM	2 x 5.0 nmol	EUR 394.8

mmu-let-7b-3p miRNA Inhibitor
MIM01005	ABM	2 x 5.0 nmol	EUR 211.2

mmu-let-7b-5p miRNA Inhibitor
MIM01006	ABM	2 x 5.0 nmol	EUR 211.2

rno-let-7d Primers
MP-r00007	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7a Primers
MP-r00033	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7c Primers
MP-r00035	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7e Primers
MP-r00036	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7f Primers
MP-r00037	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7i Primers
MP-r00038	ABM	150 ul / 10 uM	EUR 211.2

mmu-let-7a Primers
MPM00007	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7c Primers
MPM00009	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7d Primers
MPM00010	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7e Primers
MPM00011	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7f Primers
MPM00012	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7g Primers
MPM00013	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7i Primers
MPM00014	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7j Primers
MPM00753	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7k Primers
MPM00754	ABM	150 ul / 10 uM	EUR 145.2

rno-miR-7b Primers
MP-r00039	ABM	150 ul / 10 uM	EUR 211.2

mmu-miR-7b Primers
MPM00702	ABM	150 ul / 10 uM	EUR 145.2

Human pre-microRNA Expression Construct let-7b
PMIRHlet7bPA-1	SBI	Bacterial Streak	EUR 820.8

mmu-let-7a-5p Primers
MPM00736	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7c-5p Primers
MPM00741	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7d-3p Primers
MPM00742	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7d-5p Primers
MPM00743	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7e-3p Primers
MPM00744	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7e-5p Primers
MPM00745	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7f-5p Primers
MPM00748	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7g-3p Primers
MPM00749	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7g-5p Primers
MPM00750	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7i-3p Primers
MPM00751	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7i-5p Primers
MPM00752	ABM	150 ul / 10 uM	EUR 145.2

rno-let-7a-5p Primers
MPR00307	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7c-5p Primers
MPR00312	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7d-3p Primers
MPR00313	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7d-5p Primers
MPR00314	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7e-3p Primers
MPR00315	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7e-5p Primers
MPR00316	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7f-5p Primers
MPR00319	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7g-3p Primers
MPR00320	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7g-5p Primers
MPR00321	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7i-3p Primers
MPR00322	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7i-5p Primers
MPR00323	ABM	150 ul / 10 uM	EUR 211.2

Mouse pre-microRNA Expression Construct mir-7b
MMIR-7b-PA-1	SBI	Bacterial Streak	EUR 820.8

mmu-miR-7b-3p Primers
MPM02197	ABM	150 ul / 10 uM	EUR 145.2

mmu-miR-7b-5p Primers
MPM02198	ABM	150 ul / 10 uM	EUR 145.2

Dinitrophenyl (DNP)-HSA protein Conjugate
AV-9330-HSA	Alpha Diagnostics	10 mg	EUR 343.2

hsa-let-7a miRNA Antagomir
MNH01000	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7c miRNA Antagomir
MNH01007	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7d miRNA Antagomir
MNH01010	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7e miRNA Antagomir
MNH01013	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7f miRNA Antagomir
MNH01016	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7g miRNA Antagomir
MNH01020	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7i miRNA Antagomir
MNH01023	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7a miRNA Inhibitor
MIH01000	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7c miRNA Inhibitor
MIH01007	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7d miRNA Inhibitor
MIH01010	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7e miRNA Inhibitor
MIH01013	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7g miRNA Inhibitor
MIH01020	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7i miRNA Inhibitor
MIH01023	ABM	2 x 5.0 nmol	EUR 211.2

pLL3.7- Hsa- Let- 7a- 1miRNA
PVT10410	Lifescience Market	2 ug	EUR 361.2

mmu-let-7a-1-3p Primers
MPM00734	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7a-2-3p Primers
MPM00735	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7c-1-3p Primers
MPM00739	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7c-2-3p Primers
MPM00740	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7f-1-3p Primers
MPM00746	ABM	150 ul / 10 uM	EUR 145.2

mmu-let-7f-2-3p Primers
MPM00747	ABM	150 ul / 10 uM	EUR 145.2

rno-let-7a-2-3p Primers
MPR00306	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7c-1-3p Primers
MPR00310	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7c-2-3p Primers
MPR00311	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7f-1-3p Primers
MPR00317	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7f-2-3p Primers
MPR00318	ABM	150 ul / 10 uM	EUR 211.2

rno-let-7a-1-3p Primers
MPR00878	ABM	150 ul / 10 uM	EUR 211.2

hsa-let-7b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097604	Abbexa	EUR 878.40 EUR 1245.60 EUR 627.60	100 rxns 200 rxns 50 rxns

hsa-let-7a-3p miRNA Antagomir
MNH01002	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7a-5p miRNA Antagomir
MNH01003	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7c-3p miRNA Antagomir
MNH01008	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7c-5p miRNA Antagomir
MNH01009	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7d-3p miRNA Antagomir
MNH01011	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7d-5p miRNA Antagomir
MNH01012	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7e-3p miRNA Antagomir
MNH01014	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7e-5p miRNA Antagomir
MNH01015	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7f-5p miRNA Antagomir
MNH01019	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7g-3p miRNA Antagomir
MNH01021	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7g-5p miRNA Antagomir
MNH01022	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7i-3p miRNA Antagomir
MNH01024	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7i-5p miRNA Antagomir
MNH01025	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7a-3p miRNA Inhibitor
MIH01002	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7a-5p miRNA Inhibitor
MIH01003	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7c-3p miRNA Inhibitor
MIH01008	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7c-5p miRNA Inhibitor
MIH01009	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7d-3p miRNA Inhibitor
MIH01011	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7d-5p miRNA Inhibitor
MIH01012	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7e-3p miRNA Inhibitor
MIH01014	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7e-5p miRNA Inhibitor
MIH01015	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7f-5p miRNA Inhibitor
MIH01019	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7g-3p miRNA Inhibitor
MIH01021	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7g-5p miRNA Inhibitor
MIH01022	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7i-3p miRNA Inhibitor
MIH01024	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7i-5p miRNA Inhibitor
MIH01025	ABM	2 x 5.0 nmol	EUR 211.2

Mouse pre-microRNA Expression Construct let-7d
MMIR-let-7d-PA-1	SBI	Bacterial Streak	EUR 820.8

Mouse pre-microRNA Expression Construct let-7e
MMIR-let-7e-PA-1	SBI	Bacterial Streak	EUR 820.8

Mouse pre-microRNA Expression Construct let-7g
MMIR-let-7g-PA-1	SBI	Bacterial Streak	EUR 820.8

hsa-let-7a RT-PCR Detection Kit
20-abx096015	Abbexa	EUR 644.40 EUR 878.40 EUR 477.60	100 rxns 200 rxns 50 rxns

hsa-let-7c RT-PCR Detection Kit
20-abx096017	Abbexa	EUR 644.40 EUR 878.40 EUR 477.60	100 rxns 200 rxns 50 rxns

hsa-let-7d RT-PCR Detection Kit
20-abx096018	Abbexa	EUR 644.40 EUR 878.40 EUR 477.60	100 rxns 200 rxns 50 rxns

hsa-let-7e RT-PCR Detection Kit
20-abx096019	Abbexa	EUR 644.40 EUR 878.40 EUR 477.60	100 rxns 200 rxns 50 rxns

hsa-let-7f RT-PCR Detection Kit
20-abx096020	Abbexa	EUR 644.40 EUR 878.40 EUR 477.60	100 rxns 200 rxns 50 rxns

hsa-let-7g RT-PCR Detection Kit
20-abx096021	Abbexa	EUR 644.40 EUR 878.40 EUR 477.60	100 rxns 200 rxns 50 rxns

hsa-let-7i RT-PCR Detection Kit
20-abx096022	Abbexa	EUR 644.40 EUR 878.40 EUR 477.60	100 rxns 200 rxns 50 rxns

hsa-let-7f RT-PCR Detection Kit
20-abx096023	Abbexa	EUR 644.40 EUR 878.40 EUR 477.60	100 rxns 200 rxns 50 rxns

hsa-let-7a RT-PCR Primer Set
abx096769-1nmol	Abbexa	1 nmol	EUR 343.2

hsa-let-7c RT-PCR Primer Set
abx096771-1nmol	Abbexa	1 nmol	EUR 343.2

hsa-let-7d RT-PCR Primer Set
abx096772-1nmol	Abbexa	1 nmol	EUR 343.2

hsa-let-7e RT-PCR Primer Set
abx096773-1nmol	Abbexa	1 nmol	EUR 343.2

hsa-let-7f RT-PCR Primer Set
abx096774-1nmol	Abbexa	1 nmol	EUR 343.2

hsa-let-7g RT-PCR Primer Set
abx096775-1nmol	Abbexa	1 nmol	EUR 343.2

hsa-let-7i RT-PCR Primer Set
abx096776-1nmol	Abbexa	1 nmol	EUR 343.2

hsa-let-7f RT-PCR Primer Set
abx096777-1nmol	Abbexa	1 nmol	EUR 343.2

hsa-let-7a-2-3p miRNA Antagomir
MNH01001	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7f-1-3p miRNA Antagomir
MNH01017	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7f-2-3p miRNA Antagomir
MNH01018	ABM	2 x 5.0 nmol	EUR 394.8

hsa-let-7a-2-3p miRNA Inhibitor
MIH01001	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7f-1-3p miRNA Inhibitor
MIH01017	ABM	2 x 5.0 nmol	EUR 211.2

hsa-let-7f-2-3p miRNA Inhibitor
MIH01018	ABM	2 x 5.0 nmol	EUR 211.2

hsa-miR-4494 Primers
MPH02723	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-4495 Primers
MPH02724	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-4496 Primers
MPH02725	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-4497 Primers
MPH02726	ABM	150 ul / 10 uM	EUR 145.2

hsa-miR-4498 Primers
MPH02727	ABM	150 ul / 10 uM	EUR 145.2

PVS is closely related to China and isolate Iran, respectively; PAMV closely related isolates of Canada; PVH is closely related to isolates Huhhot China; and PVM is closely related to India and isolate Iran, respectively. As far as we know, PAMV in this study is the first report in Bangladesh. These findings will provide great scope for the right virus control strategies for the production of virus-free potato in Bangladesh.