A Report on Molecular Detection and Phylogenetic Evaluation of Anaplasma marginale in Ticks and Blood Samples Collected from Cattle in District Layyah in Punjab (Pakistan)

Anaplasmosis is a tick-borne disease caused by an obligate gram-negative bacteria, Anaplasma (A.) marginale. This study reports on the seasonal prevalence, epidemiology and phylogeny of A. marginale in three breeds of cattle from Layyah district, Southern Punjab, Pakistan. A total of 844 blood samples (Cross = 300, Holstein Friesian = 244, Sahiwal breed = 300) of apparently healthy cattle on a seasonal basis along with the epidemiological data collected during May 2018 to April 2019. The polymerase chain reaction resulting 265 base pair amplicons specific to a major surface 1b protein-encoding genes of A. marginale in 8.6% (73/844) of cattle registered.

The highest prevalence was observed during the fall (18.3%) followed by summer (9.7%) and winter (7.1%). Holstein Friesian breed is most susceptible to infection A. marginale (13.1%) followed by Sahiwal (7.6%) and cross-breeding (6%). partial gene sequences amplified representation of A. marginale submitted to GenBank (Accession number MK032842 and MK032843). 37/844 (4.3%) Giemsa stained blood smears found positive for Anaplasma spp. a small number of ticks including Hyalomma anatolicum, Hyalomma excavatum, Cattle Tick, Haemaphysalis punctata identified from cattle but none of them were found positive PCR for the presence of A. marginale. Epidemiological factor analysis revealed that women with cows and farm water supplies from the pool, other dairy farms where animals and dogs live with cattle and dogs have fleas burden on them had a significant association with the prevalence of A. marginale.

It was observed that the white blood cells, lymphocytes (%), monocytes (%) hematocrit, mean corpuscular hemoglobin and hemoglobin concentration of living cells on average were significantly impaired in A. marginale-positive than negative cows.

Taxonomy and Molecular phylogenetics of Ensiculiferaceae, fam. November (Peridiniales, Dinophyceae), with consideration of their life history

In the current limitation, Thoracosphaeraceae comprises all dinophytes showed calcification coccoid cells produced during the life of their history. Species hitherto assigned to Ensiculifera and Pentapharsodinium largely based on monadoid life-history stages, while links to coccoid stage (sometimes treated taxonomically different) are not always resolved. We investigate the different stages of life-history and the DNA sequence of the data Ensiculifera mexicana and other species occur in samples collected from around the world.

Based on concatenated ribosomal RNA gene sequences represented Ensiculiferaceae peridinalean different branches, which shows the relationship much to dinophytes other calcareous. Both morphological and molecular data (particularly from coccoid stage) revealed the presence of three distinct clades within Ensiculiferaceae, which may include other dinophytes showing the life-history stages of the parasite. At higher taxonomic levels, Ensiculiferaceae shows the relationship of parasites and endosymbionts (eg, Blastodinium and zooxanthella) and dinophytes save diatom not chloroplasts. These unexpected phylogenetic relationships strengthened by the presence of five plates in all taxa Cingular, which is different from the six plates cingular from most other Thoracosphaeraceae. We here describe Ensiculiferaceae, correct description and Pentapharsodinium Ensiculifera, upright Matsuokaea and give some new combinations at the species level.

A Report on Molecular Detection and Phylogenetic Evaluation of Anaplasma marginale in Ticks and Blood Samples Collected from Cattle in District Layyah in Punjab (Pakistan)
A Report on Molecular Detection and Phylogenetic Evaluation of Anaplasma marginale in Ticks and Blood Samples Collected from Cattle in District Layyah in Punjab (Pakistan)

Molecule Detection of Potato Virus in Bangladesh and their phylogenetic analysis

Potato (Solanum tuberosum) is a major food source all over the world including Bangladesh. viral diseases is a major obstacle to sustainable potato production by reducing the quality and quantity. To determine the current status of the eight important potato virus in Bangladesh, tuber samples were collected from three main potato-growing region (Munshiganj, Jessore and Bogra district) in January-February 2017 and February 2018.

The reverse transcription polymerase chain reaction (RT-PCR) and protein coat (CP) -specific primer used to amplify the CP sequence of each virus, and confirmed by sequencing, which is stored in GenBank. The results showed that the samples of tubers were subjected to virus Potato leafroll (PLRV), potato virus X (PVX), potato virus Y (PVY), virus Potato S (PVS), virus Potato H (PVH), Potato Aucuba mosaic virus (PAMV) and potato virus M (PVM) infection, whereas the mixed infections are very common. Phylogenetic analysis revealed that PLRV from this study is closely related to Canadian and Chinese isolates, respectively; PVX is closely associated with the Canadian and Chinese isolates, respectively; PVY isolates are closely related to China;

Mouse pre-microRNA Expression Construct let-7b

MMIR-let-7b-PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

rno-let-7b Primers

MP-r00034 150 ul / 10 uM
EUR 176

mmu-let-7b Primers

MPM00008 150 ul / 10 uM
EUR 121

rno-let-7b-3p Primers

MPR00308 150 ul / 10 uM
EUR 176

rno-let-7b-5p Primers

MPR00309 150 ul / 10 uM
EUR 176

mmu-let-7b-3p Primers

MPM00737 150 ul / 10 uM
EUR 121

mmu-let-7b-5p Primers

MPM00738 150 ul / 10 uM
EUR 121

hsa-let-7b miRNA Inhibitor

MIH01004 2 x 5.0 nmol
EUR 176

hsa-let-7b miRNA Antagomir

MNH01004 2 x 5.0 nmol
EUR 329

hsa-let-7a Primers

MPH01006 150 ul / 10 uM
EUR 121

hsa-let-7c Primers

MPH01008 150 ul / 10 uM
EUR 121

hsa-let-7d Primers

MPH01009 150 ul / 10 uM
EUR 121

hsa-let-7e Primers

MPH01010 150 ul / 10 uM
EUR 121

hsa-let-7f Primers

MPH01011 150 ul / 10 uM
EUR 121

hsa-let-7g Primers

MPH01012 150 ul / 10 uM
EUR 121

hsa-let-7i Primers

MPH01013 150 ul / 10 uM
EUR 121

hsa-let-7b-3p miRNA Inhibitor

MIH01005 2 x 5.0 nmol
EUR 176

hsa-let-7b-5p miRNA Inhibitor

MIH01006 2 x 5.0 nmol
EUR 176

hsa-let-7b-3p miRNA Antagomir

MNH01005 2 x 5.0 nmol
EUR 329

hsa-let-7b-5p miRNA Antagomir

MNH01006 2 x 5.0 nmol
EUR 329

hsa-let-7a-3p Primers

MPH02041 150 ul / 10 uM
EUR 121

hsa-let-7a-5p Primers

MPH02042 150 ul / 10 uM
EUR 121

hsa-let-7c-3p Primers

MPH02045 150 ul / 10 uM
EUR 121

hsa-let-7c-5p Primers

MPH02046 150 ul / 10 uM
EUR 121

hsa-let-7d-3p Primers

MPH02047 150 ul / 10 uM
EUR 121

hsa-let-7d-5p Primers

MPH02048 150 ul / 10 uM
EUR 121

hsa-let-7e-3p Primers

MPH02049 150 ul / 10 uM
EUR 121

hsa-let-7e-5p Primers

MPH02050 150 ul / 10 uM
EUR 121

hsa-let-7f-5p Primers

MPH02053 150 ul / 10 uM
EUR 121

hsa-let-7g-3p Primers

MPH02054 150 ul / 10 uM
EUR 121

hsa-let-7g-5p Primers

MPH02055 150 ul / 10 uM
EUR 121

hsa-let-7i-3p Primers

MPH02056 150 ul / 10 uM
EUR 121

hsa-let-7i-5p Primers

MPH02057 150 ul / 10 uM
EUR 121

hsa-let-7b RT-PCR Detection Kit

20-abx096016
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

hsa-let-7b RT-PCR Primer Set

abx096770-1nmol 1 nmol
EUR 286
  • Shipped within 5-10 working days.

hsa-let-7f-1-3p Primers

MPH02051 150 ul / 10 uM
EUR 121

hsa-let-7f-2-3p Primers

MPH02052 150 ul / 10 uM
EUR 121

hsa-let-7a-2-3p Primers

MPH03980 150 ul / 10 uM
EUR 121

mmu-let-7b miRNA Antagomir

MNM01004 2 x 5.0 nmol
EUR 329

rno-let-7b miRNA Antagomir

MNR01004 2 x 5.0 nmol
EUR 329

mmu-let-7b miRNA Inhibitor

MIM01004 2 x 5.0 nmol
EUR 176

rno-let-7b miRNA Inhibitor

MIR01004 2 x 5.0 nmol
EUR 176

hsa-let-7b RT-PCR Detection and U6 Calibration Kit

20-abx096393
  • EUR 690.00
  • EUR 954.00
  • EUR 495.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

mmu-let-7b-3p miRNA Antagomir

MNM01005 2 x 5.0 nmol
EUR 329

mmu-let-7b-5p miRNA Antagomir

MNM01006 2 x 5.0 nmol
EUR 329

rno-let-7b-3p miRNA Antagomir

MNR01005 2 x 5.0 nmol
EUR 329

rno-let-7b-5p miRNA Antagomir

MNR01006 2 x 5.0 nmol
EUR 329

mmu-let-7b-3p miRNA Inhibitor

MIM01005 2 x 5.0 nmol
EUR 176

mmu-let-7b-5p miRNA Inhibitor

MIM01006 2 x 5.0 nmol
EUR 176

rno-let-7b-3p miRNA Inhibitor

MIR01005 2 x 5.0 nmol
EUR 176

rno-let-7b-5p miRNA Inhibitor

MIR01006 2 x 5.0 nmol
EUR 176

rno-let-7d Primers

MP-r00007 150 ul / 10 uM
EUR 176

rno-let-7a Primers

MP-r00033 150 ul / 10 uM
EUR 176

rno-let-7c Primers

MP-r00035 150 ul / 10 uM
EUR 176

rno-let-7e Primers

MP-r00036 150 ul / 10 uM
EUR 176

rno-let-7f Primers

MP-r00037 150 ul / 10 uM
EUR 176

rno-let-7i Primers

MP-r00038 150 ul / 10 uM
EUR 176

mmu-let-7a Primers

MPM00007 150 ul / 10 uM
EUR 121

mmu-let-7c Primers

MPM00009 150 ul / 10 uM
EUR 121

mmu-let-7d Primers

MPM00010 150 ul / 10 uM
EUR 121

mmu-let-7e Primers

MPM00011 150 ul / 10 uM
EUR 121

mmu-let-7f Primers

MPM00012 150 ul / 10 uM
EUR 121

mmu-let-7g Primers

MPM00013 150 ul / 10 uM
EUR 121

mmu-let-7i Primers

MPM00014 150 ul / 10 uM
EUR 121

mmu-let-7j Primers

MPM00753 150 ul / 10 uM
EUR 121

mmu-let-7k Primers

MPM00754 150 ul / 10 uM
EUR 121

rno-miR-7b Primers

MP-r00039 150 ul / 10 uM
EUR 176

mmu-miR-7b Primers

MPM00702 150 ul / 10 uM
EUR 121

Human pre-microRNA Expression Construct let-7b

PMIRHlet7bPA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

rno-let-7a-5p Primers

MPR00307 150 ul / 10 uM
EUR 176

rno-let-7c-5p Primers

MPR00312 150 ul / 10 uM
EUR 176

rno-let-7d-3p Primers

MPR00313 150 ul / 10 uM
EUR 176

rno-let-7d-5p Primers

MPR00314 150 ul / 10 uM
EUR 176

rno-let-7e-3p Primers

MPR00315 150 ul / 10 uM
EUR 176

rno-let-7e-5p Primers

MPR00316 150 ul / 10 uM
EUR 176

rno-let-7f-5p Primers

MPR00319 150 ul / 10 uM
EUR 176

rno-let-7g-3p Primers

MPR00320 150 ul / 10 uM
EUR 176

rno-let-7g-5p Primers

MPR00321 150 ul / 10 uM
EUR 176

rno-let-7i-3p Primers

MPR00322 150 ul / 10 uM
EUR 176

rno-let-7i-5p Primers

MPR00323 150 ul / 10 uM
EUR 176

mmu-let-7a-5p Primers

MPM00736 150 ul / 10 uM
EUR 121

mmu-let-7c-5p Primers

MPM00741 150 ul / 10 uM
EUR 121

mmu-let-7d-3p Primers

MPM00742 150 ul / 10 uM
EUR 121

mmu-let-7d-5p Primers

MPM00743 150 ul / 10 uM
EUR 121

mmu-let-7e-3p Primers

MPM00744 150 ul / 10 uM
EUR 121

mmu-let-7e-5p Primers

MPM00745 150 ul / 10 uM
EUR 121

mmu-let-7f-5p Primers

MPM00748 150 ul / 10 uM
EUR 121

mmu-let-7g-3p Primers

MPM00749 150 ul / 10 uM
EUR 121

mmu-let-7g-5p Primers

MPM00750 150 ul / 10 uM
EUR 121

mmu-let-7i-3p Primers

MPM00751 150 ul / 10 uM
EUR 121

mmu-let-7i-5p Primers

MPM00752 150 ul / 10 uM
EUR 121

Mouse pre-microRNA Expression Construct mir-7b

MMIR-7b-PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

mmu-miR-7b-3p Primers

MPM02197 150 ul / 10 uM
EUR 121

mmu-miR-7b-5p Primers

MPM02198 150 ul / 10 uM
EUR 121

Dinitrophenyl (DNP)-HSA protein Conjugate

AV-9330-HSA 10 mg
EUR 286

hsa-let-7a miRNA Inhibitor

MIH01000 2 x 5.0 nmol
EUR 176

hsa-let-7c miRNA Inhibitor

MIH01007 2 x 5.0 nmol
EUR 176

hsa-let-7d miRNA Inhibitor

MIH01010 2 x 5.0 nmol
EUR 176

hsa-let-7e miRNA Inhibitor

MIH01013 2 x 5.0 nmol
EUR 176

hsa-let-7g miRNA Inhibitor

MIH01020 2 x 5.0 nmol
EUR 176

hsa-let-7i miRNA Inhibitor

MIH01023 2 x 5.0 nmol
EUR 176

hsa-let-7a miRNA Antagomir

MNH01000 2 x 5.0 nmol
EUR 329

hsa-let-7c miRNA Antagomir

MNH01007 2 x 5.0 nmol
EUR 329

hsa-let-7d miRNA Antagomir

MNH01010 2 x 5.0 nmol
EUR 329

hsa-let-7e miRNA Antagomir

MNH01013 2 x 5.0 nmol
EUR 329

hsa-let-7f miRNA Antagomir

MNH01016 2 x 5.0 nmol
EUR 329

hsa-let-7g miRNA Antagomir

MNH01020 2 x 5.0 nmol
EUR 329

hsa-let-7i miRNA Antagomir

MNH01023 2 x 5.0 nmol
EUR 329

pLL3.7- Hsa- Let- 7a- 1miRNA

PVT10410 2 ug
EUR 301

rno-let-7a-2-3p Primers

MPR00306 150 ul / 10 uM
EUR 176

rno-let-7c-1-3p Primers

MPR00310 150 ul / 10 uM
EUR 176

rno-let-7c-2-3p Primers

MPR00311 150 ul / 10 uM
EUR 176

rno-let-7f-1-3p Primers

MPR00317 150 ul / 10 uM
EUR 176

rno-let-7f-2-3p Primers

MPR00318 150 ul / 10 uM
EUR 176

rno-let-7a-1-3p Primers

MPR00878 150 ul / 10 uM
EUR 176

mmu-let-7a-1-3p Primers

MPM00734 150 ul / 10 uM
EUR 121

mmu-let-7a-2-3p Primers

MPM00735 150 ul / 10 uM
EUR 121

mmu-let-7c-1-3p Primers

MPM00739 150 ul / 10 uM
EUR 121

mmu-let-7c-2-3p Primers

MPM00740 150 ul / 10 uM
EUR 121

mmu-let-7f-1-3p Primers

MPM00746 150 ul / 10 uM
EUR 121

mmu-let-7f-2-3p Primers

MPM00747 150 ul / 10 uM
EUR 121

hsa-let-7b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097604
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-let-7a-3p miRNA Inhibitor

MIH01002 2 x 5.0 nmol
EUR 176

hsa-let-7a-5p miRNA Inhibitor

MIH01003 2 x 5.0 nmol
EUR 176

hsa-let-7c-3p miRNA Inhibitor

MIH01008 2 x 5.0 nmol
EUR 176

hsa-let-7c-5p miRNA Inhibitor

MIH01009 2 x 5.0 nmol
EUR 176

hsa-let-7d-3p miRNA Inhibitor

MIH01011 2 x 5.0 nmol
EUR 176

hsa-let-7d-5p miRNA Inhibitor

MIH01012 2 x 5.0 nmol
EUR 176

hsa-let-7e-3p miRNA Inhibitor

MIH01014 2 x 5.0 nmol
EUR 176

hsa-let-7e-5p miRNA Inhibitor

MIH01015 2 x 5.0 nmol
EUR 176

hsa-let-7f-5p miRNA Inhibitor

MIH01019 2 x 5.0 nmol
EUR 176

hsa-let-7g-3p miRNA Inhibitor

MIH01021 2 x 5.0 nmol
EUR 176

hsa-let-7g-5p miRNA Inhibitor

MIH01022 2 x 5.0 nmol
EUR 176

hsa-let-7i-3p miRNA Inhibitor

MIH01024 2 x 5.0 nmol
EUR 176

hsa-let-7i-5p miRNA Inhibitor

MIH01025 2 x 5.0 nmol
EUR 176

hsa-let-7a-3p miRNA Antagomir

MNH01002 2 x 5.0 nmol
EUR 329

hsa-let-7a-5p miRNA Antagomir

MNH01003 2 x 5.0 nmol
EUR 329

hsa-let-7c-3p miRNA Antagomir

MNH01008 2 x 5.0 nmol
EUR 329

hsa-let-7c-5p miRNA Antagomir

MNH01009 2 x 5.0 nmol
EUR 329

hsa-let-7d-3p miRNA Antagomir

MNH01011 2 x 5.0 nmol
EUR 329

hsa-let-7d-5p miRNA Antagomir

MNH01012 2 x 5.0 nmol
EUR 329

hsa-let-7e-3p miRNA Antagomir

MNH01014 2 x 5.0 nmol
EUR 329

hsa-let-7e-5p miRNA Antagomir

MNH01015 2 x 5.0 nmol
EUR 329

hsa-let-7f-5p miRNA Antagomir

MNH01019 2 x 5.0 nmol
EUR 329

hsa-let-7g-3p miRNA Antagomir

MNH01021 2 x 5.0 nmol
EUR 329

hsa-let-7g-5p miRNA Antagomir

MNH01022 2 x 5.0 nmol
EUR 329

hsa-let-7i-3p miRNA Antagomir

MNH01024 2 x 5.0 nmol
EUR 329

hsa-let-7i-5p miRNA Antagomir

MNH01025 2 x 5.0 nmol
EUR 329

Mouse pre-microRNA Expression Construct let-7d

MMIR-let-7d-PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

Mouse pre-microRNA Expression Construct let-7e

MMIR-let-7e-PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

Mouse pre-microRNA Expression Construct let-7g

MMIR-let-7g-PA-1 Bacterial Streak
EUR 684
  • Category: MicroRNA Tools

hsa-let-7a RT-PCR Detection Kit

20-abx096015
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

hsa-let-7c RT-PCR Detection Kit

20-abx096017
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

hsa-let-7d RT-PCR Detection Kit

20-abx096018
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

hsa-let-7e RT-PCR Detection Kit

20-abx096019
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

hsa-let-7f RT-PCR Detection Kit

20-abx096020
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

hsa-let-7g RT-PCR Detection Kit

20-abx096021
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

hsa-let-7i RT-PCR Detection Kit

20-abx096022
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

hsa-let-7f RT-PCR Detection Kit

20-abx096023
  • EUR 537.00
  • EUR 732.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

hsa-let-7a RT-PCR Primer Set

abx096769-1nmol 1 nmol
EUR 286
  • Shipped within 5-10 working days.

hsa-let-7c RT-PCR Primer Set

abx096771-1nmol 1 nmol
EUR 286
  • Shipped within 5-10 working days.

hsa-let-7d RT-PCR Primer Set

abx096772-1nmol 1 nmol
EUR 286
  • Shipped within 5-10 working days.

hsa-let-7e RT-PCR Primer Set

abx096773-1nmol 1 nmol
EUR 286
  • Shipped within 5-10 working days.

hsa-let-7f RT-PCR Primer Set

abx096774-1nmol 1 nmol
EUR 286
  • Shipped within 5-10 working days.

hsa-let-7g RT-PCR Primer Set

abx096775-1nmol 1 nmol
EUR 286
  • Shipped within 5-10 working days.

hsa-let-7i RT-PCR Primer Set

abx096776-1nmol 1 nmol
EUR 286
  • Shipped within 5-10 working days.

hsa-let-7f RT-PCR Primer Set

abx096777-1nmol 1 nmol
EUR 286
  • Shipped within 5-10 working days.

hsa-let-7a-2-3p miRNA Inhibitor

MIH01001 2 x 5.0 nmol
EUR 176

hsa-let-7f-1-3p miRNA Inhibitor

MIH01017 2 x 5.0 nmol
EUR 176

hsa-let-7f-2-3p miRNA Inhibitor

MIH01018 2 x 5.0 nmol
EUR 176

hsa-let-7a-2-3p miRNA Antagomir

MNH01001 2 x 5.0 nmol
EUR 329

hsa-let-7f-1-3p miRNA Antagomir

MNH01017 2 x 5.0 nmol
EUR 329

hsa-let-7f-2-3p miRNA Antagomir

MNH01018 2 x 5.0 nmol
EUR 329

hsa-miR-1 Primers

MPH01014 150 ul / 10 uM
EUR 121

hsa-miR-100 Primers

MPH01015 150 ul / 10 uM
EUR 121

PVS is closely related to China and isolate Iran, respectively; PAMV closely related isolates of Canada; PVH is closely related to isolates Huhhot China; and PVM is closely related to India and isolate Iran, respectively. As far as we know, PAMV in this study is the first report in Bangladesh. These findings will provide great scope for the right virus control strategies for the production of virus-free potato in Bangladesh.

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